Results 31 to 40 of about 103,477 (279)

Allele quantification using molecular inversion probes (MIP) [PDF]

Nucleic Acids Research, 2005
Detection of genomic copy number changes has been an important research area, especially in cancer. Several high-throughput technologies have been developed to detect these changes. Features that are important for the utility of technologies assessing copy number changes include the ability to interrogate regions of interest at the desired density as ...
Wang, Yuker, Moorhead, Martin, Karlin-Neumann, George, Falkowski, Matthew, Chen, Chunnuan, Siddiqui, Farooq, Davis, Ronald W., Willis, Thomas D., Faham, Malek   +8 more
openaire   +2 more sources

Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications. [PDF]

PLoS ONE, 2007
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system.
Michael S Akhras, Magnus Unemo, Sreedevi Thiyagarajan, Pål Nyrén, Ronald W Davis, Andrew Z Fire, Nader Pourmand   +6 more
doaj   +1 more source

BRCA Testing by Single-Molecule Molecular Inversion Probes [PDF]

Clinical Chemistry, 2017
Abstract BACKGROUND Despite advances in next generation DNA sequencing (NGS), NGS-based single gene tests for diagnostic purposes require improvements in terms of completeness, quality, speed, and cost.
Neveling, K., Mensenkamp, A.R., Derks, R, Kwint, M.P., Ouchene, H., Steehouwer, M., van Lier, L.A., Bosgoed, E.A.J., Rikken, A., Tychon, M.W.J., Zafeiropoulou, D., Castelein, S., Hehir-Kwa, J.Y., Thung, G.W., Hofste, T., Lelieveld, S.H., Bertens, S.M., Adan, I.B., Eijkelenboom, A., Tops, B.B.J., Yntema, H.G., Stokowy, T., Knappskog, P.M., Hoberg-Vetti, H., Steen, V.M., Boyle, E., Martin, B., Ligtenberg, M.J.L., Ligtenberg, M.J.L., Shendure, J., Nelen, M.R., Hoischen, A., Hoischen, A.   +32 more
openaire   +2 more sources

Analysis of molecular inversion probe performance for allele copy number determination [PDF]

Genome Biology, 2007
AbstractWe have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 μg) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude ...
Wang, Yuker, Moorhead, Martin, Karlin-Neumann, George, Wang, Nicholas J, Ireland, James, Lin, Steven, Chen, Chunnuan, Heiser, Laura M, Chin, Koei, Esserman, Laura, Gray, Joe W, Spellman, Paul T, Faham, Malek   +12 more
openaire   +6 more sources

Development of Molecular Inversion Probes for Soybean Progeny Genomic Selection Genotyping

The Plant Genome, 2022
AbstractIncreasing rate of genetic gain for key agronomic traits through genomic selection requires the development of new molecular methods to run genome-wide single nucleotide polymorphisms (SNPs). The main limitation of current methods is the cost is too high to screen breeding populations.
Haichuan Wang, Benjamin Campbell, Mary Happ, Samantha McConaughy, Aaron Lorenz, Keenan Amundsen, Qijian Song, Vincent Pantalone, David Hyten   +8 more
openaire   +3 more sources

PhenoMIP: High-Throughput Phenotyping of Diverse Caenorhabditis elegans Populations via Molecular Inversion Probes [PDF]

G3 Genes|Genomes|Genetics, 2020
Abstract Whether generated within a lab setting or isolated from the wild, variant alleles continue to be an important resource for decoding gene function in model organisms such as Caenorhabditis elegans. With advances in massively parallel sequencing, multiple whole-genome sequenced (WGS) strain collections are now available to the ...
Calvin Mok, Gabriella Belmarez, Mark L. Edgley, Donald G. Moerman, Robert H. Waterston   +4 more
openaire   +4 more sources

PathogenMip assay: a multiplex pathogen detection assay. [PDF]

PLoS ONE, 2007
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection.
Michael S Akhras, Sreedevi Thiyagarajan, Andrea C Villablanca, Ronald W Davis, Pål Nyrén, Nader Pourmand   +5 more
doaj   +1 more source

A Genetics-First Approach Revealed Monogenic Disorders in Patients With ARM and VACTERL Anomalies

Frontiers in Pediatrics, 2020
Background: The VATER/VACTERL association (VACTERL) is defined as the non-random occurrence of the following congenital anomalies: Vertebral, Anal, Cardiac, Tracheal-Esophageal, Renal, and Limb anomalies.
Romy van de Putte, Gabriel C. Dworschak, Gabriel C. Dworschak, Erwin Brosens, Erwin Brosens, Heiko M. Reutter, Heiko M. Reutter, Carlo L. M. Marcelis, Rocio Acuna-Hidalgo, Nehir E. Kurtas, Marloes Steehouwer, Sally L. Dunwoodie, Eberhard Schmiedeke, Stefanie Märzheuser, Nicole Schwarzer, Alice S. Brooks, Annelies de Klein, Cornelius E. J. Sloots, Dick Tibboel, Giulia Brisighelli, Giulia Brisighelli, Anna Morandi, Maria F. Bedeschi, Michael D. Bates, Michael D. Bates, Marc A. Levitt, Marc A. Levitt, Marc A. Levitt, Alberto Peña, Alberto Peña, Alberto Peña, Ivo de Blaauw, Nel Roeleveld, Han G. Brunner, Han G. Brunner, Iris A. L. M. van Rooij, Alexander Hoischen, Alexander Hoischen   +37 more
doaj   +1 more source

Inversion doublets of reflection-asymmetric clustering in 28Si and their isoscalar monopole and dipole transitions [PDF]

, 2016
[Background] Various cluster states of astrophysical interest are expected to exist in the excited states of $^{28}{\rm Si}$. However, they have not been identified firmly, because of the experimental and theoretical difficulties.
Chiba, Y., Kimura, M., Taniguchi, Y.
core   +2 more sources

Phylogenetic inferences of Atelinae (Platyrrhini) based on multi-directional chromosome painting in Brachyteles arachnoides, Ateles paniscus paniscus and Ateles b. marginatus [PDF]

, 2005
We performed multi-directional chromosome painting in a comparative cytogenetic study of the three Atelinae species Brachyteles arachnoides, Ateles paniscus paniscus and Ateles belzebuth marginatus, in order to reconstruct phylogenetic relationships ...
C. Nagamachi, Collins AC, Consigliere S, Dutrillaux B, Dutrillaux B, E.H.C. de Oliveira, Garcia F, Garcia F, I.J. Sbalqueiro, J.C. Pieczarka, Kay RF, Kellogg R, Kunkel LM, M. Neusser, Muller S, Pequignot E, Pieczarka JC, S. Müller   +17 more
core   +1 more source