Low-cost equipment for electroformation of Giant Unilamellar Vesicles [PDF]
HardwareX, 2018 Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium
Javier Alejandro Bellon +2 more
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Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles. [PDF]
PLoS ONE, 2011 Voltage-gated ion channels are key players in cellular excitability. Recent studies suggest that their behavior can depend strongly on the membrane lipid composition and physical state. In vivo studies of membrane/channel and channel/channel interactions
Sophie Aimon +5 more
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Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels [PDF]
Bio-Protocol, 2020 Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics.
Eric Parigoris +2 more
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Osmotic Gradients Induce Bio-reminiscent Morphological Transformations in Giant Unilamellar Vesicles [PDF]
Frontiers in Physiology, 2012 We report observations of large-scale, in-plane and out-of-plane membrane deformations in giant uni- and multilamellar vesicles composed of binary and ternary lipid mixtures in the presence of net transvesicular osmotic gradients.
Kamila eOglecka +4 more
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Law of corresponding states for osmotic swelling of vesicles [PDF]
Soft Matter, 2012; 8: 2185-2193, 2011 As solute molecules permeate into a vesicle due to a concentration difference across its membrane, the vesicle swells through osmosis. The swelling can be divided into two stages: (a) an "ironing" stage, where the volume-to-area ratio of the vesicle increases without a significant change in its area; (b) a stretching stage, where the vesicle grows ...
Angelova +32 more
arxiv +3 more sources
Microfluidic production, stability and loading of synthetic giant unilamellar vesicles [PDF]
Scientific ReportsIn advanced drug delivery, versatile liposomal formulations are commonly employed for safer and more accurate therapies. Here we report a method that allows a straightforward production of synthetic monodisperse (~ 100 μm) giant unilamellar vesicles ...
Mart Ernits +11 more
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Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content [PDF]
MembranesGiant unilamellar vesicles (GUVs) are membrane models used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol, dry lipid film is formed.
Ivan Mardešić +2 more
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Dataset of asymmetric giant unilamellar vesicles prepared via hemifusion: Observation of anti-alignment of domains and modulated phases in asymmetric bilayers. [PDF]
Data in Brief, 2021 The data provided with this paper are confocal fluorescence images of symmetric giant unilamellar vesicles (GUVs) and asymmetric giant unilamellar vesicles (aGUVs).
Thais A. Enoki +3 more
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Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference [PDF]
Life, 2021 The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems.
Ylenia Miele +3 more
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Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter [PDF]
Scientific Reports, 2021 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate a rapid bi-directional movement of lipids without metabolic energy input. Here, we established a
Patricia P. M. Mathiassen +2 more
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