Engineering Phosphatidylserine Containing Asymmetric Giant Unilamellar Vesicles [PDF]
MembranesThe plasma membrane lipid distribution is asymmetric, with several anionic lipid species located in its inner leaflet. Among these, phosphatidylserine (PS) plays a crucial role in various important physiological functions.
Jake McDonough +5 more
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Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles [PDF]
Pharmaceutics, 2022 In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Paola Albanese +6 more
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A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles [PDF]
Bio-Protocol, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Patricia Mathiassen +1 more
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Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles [PDF]
Molecules, 2022 Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Ogadimma D. Okagu +2 more
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Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles [PDF]
ACS OmegaWe demonstrate rapid membrane mixing between GUVs of pure lipid compositions and membrane vesicles (MVs) isolated from the plasma membrane of Vero cells, resulting in the transfer of native lipids and proteins to the GUVs. The steps involved in the membrane mixing are docking followed by membrane fusion.
Garvita Dhanawat +3 more
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Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles [PDF]
Advanced Science, 2023 In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Jorik Waeterschoot +5 more
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Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids. [PDF]
PLoS ONE, 2015 Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion.
Soichiro Tsuda +4 more
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Effects of membrane potentials on the electroporation of giant unilamellar vesicles. [PDF]
PLoS ONE, 2023 Living organisms maintain a resting membrane potential, which plays an important role in various biophysical and biological processes. In the context of medical applications, irreversible electroporation (IRE) is a non-thermal and minimally invasive ...
Md Abdul Wadud +3 more
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Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics [PDF]
Communications Chemistry, 2021 The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives.
Naresh Yandrapalli +3 more
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A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets [PDF]
Membranes, 2023 Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells.
Yiting Zhang, Haruto Obuchi, Taro Toyota
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