Results 1 to 10 of about 8,544,371 (366)

High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
doaj   +1 more source

High-resolution mass spectrometry of small molecules bound to membrane proteins

Nature Methods, 2016
Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define.
J. Gault, J. Donlan, I. Liko, Jonathan T S Hopper, Kallol Gupta, N. G. Housden, W. Struwe, M. Marty, T. Mize, Cherine Bechara, Ya Zhu, Beili Wu, C. Kleanthous, M. Belov, Eugen Damoc, A. Makarov, C. Robinson   +16 more
semanticscholar   +1 more source

Modeling the Membrane Environment for Membrane Proteins [PDF]

Biophysical Journal, 2011
Increasingly, the membrane protein community is recognizing the sensitivity of protein structure and function to the protein's environment. Hofer et al. (1), in their recent Letter in the Biophysical Journal, aim to develop a crystallization protocol that will have increased biological relevance.
Timothy A. Cross, J. Antoinette Killian, David D. Busath, Myriam Cotten, Frances Separovic   +4 more
openaire   +2 more sources

Reagents and models for detecting endogenous GLP1R and GIPR

EBioMedicine, 2021
Glucagon-like peptide-1 receptor (GLP1R) agonists target the GLP1R, whereas dual GLP1R/ gastric inhibitory polypeptide receptor (GIPR) agonists target both the GLP1R and GIPR. Despite the importance of these drug classes for the treatment of diabetes and
Julia Ast, Johannes Broichhagen, David J. Hodson   +2 more
doaj  

Author Correction: Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Nature Communications, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Julia Ast, Anastasia Arvaniti, Nicholas H. F. Fine, Daniela Nasteska, Fiona B. Ashford, Zania Stamataki, Zsombor Koszegi, Andrea Bacon, Ben J. Jones, Maria A. Lucey, Shugo Sasaki, Daniel I. Brierley, Benoit Hastoy, Alejandra Tomas, Giuseppe D’Agostino, Frank Reimann, Francis C. Lynn, Christopher A. Reissaus, Amelia K. Linnemann, Elisa D’Este, Davide Calebiro, Stefan Trapp, Kai Johnsson, Tom Podewin, Johannes Broichhagen, David J. Hodson   +25 more
doaj   +1 more source

Spontaneous Suppressors against Debilitating Transmembrane Mutants of CaMdr1 Disclose Novel Interdomain Communication via Signature Motifs of the Major Facilitator Superfamily

Journal of Fungi, 2022
The Major Facilitator Superfamily (MFS) drug:H+ antiporter CaMdr1, from Candida albicans, is responsible for the efflux of structurally diverse antifungals.
Suman Sharma, Atanu Banerjee, Alexis Moreno, Archana Kumari Redhu, Pierre Falson, Rajendra Prasad   +5 more
doaj   +1 more source

Circular dichroism spectroscopy of membrane proteins.

Chemical Society Reviews, 2016
Circular dichroism (CD) spectroscopy is a well-established technique for studying the secondary structures, dynamics, folding pathways, and interactions of soluble proteins, and is complementary to the high resolution but generally static structures ...
Andrew J. Miles, Bonnie A. Wallace
semanticscholar   +1 more source

Directed Mutational Strategies Reveal Drug Binding and Transport by the MDR Transporters of Candida albicans

Journal of Fungi, 2021
Multidrug resistance (MDR) transporters belonging to either the ATP-Binding Cassette (ABC) or Major Facilitator Superfamily (MFS) groups are major determinants of clinical drug resistance in fungi.
Atanu Banerjee, Jorgaq Pata, Suman Sharma, Brian C. Monk, Pierre Falson, Rajendra Prasad   +5 more
doaj   +1 more source

SOSUI: classification and secondary structure prediction system for membrane proteins

Bioinform., 1998
UNLABELLED The system SOSUI for the discrimination of membrane proteins and soluble ones together with the prediction of transmembrane helices was developed, in which the accuracy of the classification of proteins was 99% and the corresponding value for ...
T. Hirokawa, Seah Boon-Chieng, S. Mitaku
semanticscholar   +1 more source

Membrane systems with proteins embedded in membranes

Theoretical Computer Science, 2008
Membrane computing is a biologically inspired computational paradigm. Motivated by brane calculi we investigate membrane systems which differ from conventional membrane systems by the following features: (1) biomolecules (proteins) can move through the regions of the systems, and can attach onto (and de-attach from) membranes, and (2 ...
Brijder R., Cavaliere M., Riscos-Núñez A., Rozenberg G., Sburlan D.   +4 more
openaire   +3 more sources