Results 181 to 190 of about 5,652,303 (213)

Unleashing phosphorus mononitride. [PDF]

Nat Commun
Edin S, Sandoval-Pauker C, Yutronkie NJ, Takacs Z, Wilhelm F, Rogalev A, Pinter B, Pedersen KS, Reinholdt A.   +8 more
europepmc   +1 more source

Short peptide perturbs spermatogenesis via immune microenvironment dysregulation and mitochondrial imbalance

FEBS Open Bio, EarlyView.
In the blood–testis barrier, occludin is crucial for tight junctions. This study demonstrates that occludin‐targeting short peptides disrupt junction integrity, inducing immune cell infiltration, tumor necrosis factor‐α/interleukin‐6 secretion and mitochondrial dysfunction, ultimately triggering apoptosis.
Heng Wang, Xiaofang Tan, Deyu Chen
wiley   +1 more source

Genomic Prediction and Heritability Estimation for Daughter Pregnancy Rate in U.S. Holstein Cows Using SNP, Epistasis and Haplotype Effects. [PDF]

Int J Mol Sci
Yang R, Prakapenka D, Liang Z, Da Y.
europepmc   +1 more source

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

Revisiting D-Acylases for D-Amino Acid Production. [PDF]

Microb Biotechnol
Martínez-Rodríguez S, Gavira JA.
europepmc   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Identifying lifelong factors that impact brain health and functional outcomes in adults with childhood-onset type 1 diabetes: the cognition and longitudinal assessments of risk factors over 30 years (CLARiFY) - diabetes complications study protocol. [PDF]

BMJ Open
Brown A, Sakowski SA, Moran C, Koubek EJ, Srikanth VK, Zhao L, Collyer T, Adamson CL, Northam EA, Beare R, Cameron FJ, Feldman EL.   +11 more
europepmc   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Urinary Metabolic Biomarkers of Attentional Control in Children With Attention-Deficit/Hyperactivity Disorder: A Dimensional Approach Through ¹H NMR-Based Metabolomics. [PDF]

NMR Biomed
Salmerón ADM, Fernández-Martín P, Rodríguez-Herrera R, Arrabal-Campos FM, Abreu AC, Fernández I, Flores P.   +6 more
europepmc   +1 more source