FEBS Open Bio, EarlyView.Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Serum 25 hydroxyvitamin D was positively associated with hemoglobin and other anemia parameters in children: a cross-sectional study. [PDF]
Nutr Metab (Lond)Dong H, Xu X, Gao L, Ni Y.
europepmc +1 more source
Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli
FEBS Open Bio, EarlyView.BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa +2 more
wiley +1 more source
LymphSens study: the enigma of subjective lymphoedema - how often and why do patients report lymphoedema after breast cancer treatment without an objective measurable swelling? The role of lymphatic and sensory processing problems: a protocol for a multicentre prospective longitudinal study. [PDF]
BMJ OpenGursen C +14 more
europepmc +1 more source
Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase
FEBS Open Bio, EarlyView.The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni +4 more
wiley +1 more source
Differential expression of cytokines and vitamin D in benign and malignant thyroid diseases. [PDF]
Sci RepLi H, Jin X, Zhang M, Wang X, Wang H.
europepmc +1 more source
Matrix: a complex amalgam of structures and functions in tumor microenvironment
FEBS Open Bio, EarlyView.The matrix is a dynamic, intricate three‐dimensional mesh of biomolecules with both structural and functional properties. This review deals with the complexity of this ‘molecular amalgam’ in the tumor microenvironment and highlights its importance in the maintenance and evolution of tumors by describing certain matrix biomolecules, such as ...
Spyros S. Skandalis +3 more
wiley +1 more source
Corrigendum: Co-administration of vitamin D and N-acetylcysteine to modulate immunosenescence in older adults with vitamin D deficiency: a randomized clinical trial. [PDF]
Front ImmunolRastgoo S +4 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Phenotypic plasticity in a newly established set of EGFR inhibitor‐adapted NSCLC cell lines during adaptation and in established cell lines. Here, we introduce novel sublines of the EGFR‐mutant non‐small cell lung cancer (NSCLC) cell lines HCC827 and HCC4006 adapted to the EGFR kinase inhibitors gefitinib (HCC827rGEFI2μm, HCC4006rGEFI1μm), erlotinib ...
Tharsagini V. Nanthaprakash +6 more
wiley +1 more source