Results 281 to 290 of about 8,344,499 (308)

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

A systematic review and meta-analysis of circulating 25-hydroxyvitamin D concentration and vitamin D status worldwide. [PDF]

J Public Health (Oxf)
Dunlop E, Pham NM, Van Hoang D, Mazahery H, Neo B, Shrapnel J, Kalmpourtzidou A, Chai KEK, Ng L, Black LJ.   +9 more
europepmc   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Correction: Phytochemical profile and antioxidant activity of Dracocephalum kotschyi boiss. Affected by environmental conditions of cultivation regions). [PDF]

BMC Plant Biol
Zamani S, Bakhshi D, Ebadi MT, Sahraroo A.   +3 more
europepmc   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Vitamin D Levels During Pregnancy and Dental Caries in Offspring.

JAMA Netw Open
Xu N, Chen Z, Wang B, Qiu Y, Alifu X, Zhou H, Cheng H, Huang Y, Zhang L, Liu H, Yu L, Chen D, Yu Y.   +12 more
europepmc   +1 more source

Associations of bone health biomarkers and bone mineral density with dietary intakes in vitamin D deficient women: a cross-sectional study. [PDF]

Sci Rep
Peris CH, Selvam S, Swaminathan S, Rangarajan R, Raj T, Thankachan P.   +5 more
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source