Results 181 to 190 of about 5,652,315 (212)

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

open access: yesFEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo   +4 more
wiley   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

<i>Rotavirus alphagastroenteritidis</i>: Circulating Strains After the Introduction of the Rotavirus Vaccine (Rotarix<sup>®</sup>) in Luanda Province of Angola. [PDF]

open access: yesViruses
Vita DG   +9 more
europepmc   +1 more source

Revisiting D-Acylases for D-Amino Acid Production. [PDF]

open access: yesMicrob Biotechnol
Martínez-Rodríguez S, Gavira JA.
europepmc   +1 more source

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