Results 31 to 40 of about 2,148 (81)

有和无甘油的聚丙烯酰胺胶在检测突变时的差别

四川大学学报. 自然科学版, 2000
有文献报道 ,在非变性的聚丙烯酰胺中加入甘油可提高SSCP检测的灵敏度 .作者采用PCR SSCP、加 5%的甘油或未加甘油的 10 %非变性聚丙烯酰胺胶和DNA测序技术 ,来检测常染色体显性多囊肾病致病基因PKD1的突变 .结果发现 ,同一个病人在未加甘油的10 %非变性聚丙烯酰胺胶中 ,有 4条单链带 ,而在加 5%的甘油的 10 %非变性聚丙烯酰胺胶中仅有两条单链带 ;两种胶上均有迁移率较正常双链带慢一条的双链带 .测序结果显示 ,在有异常迁移率带的个体的 1条DNA分子中缺失 1个G ...
丁兰, 张思仲, 周宏远, 邱为民, 肖翠英   +4 more
doaj  

不同土壤管理措施下坡耕地产流产沙和氮磷流失特征

Shuitu Baochi Xuebao, 2016
采用野外径流小区定位观测法，研究次降雨条件下腐殖酸（HA）、聚丙烯酰胺（PAM）、绿肥（GM）3种土壤管理措施对紫色丘陵区坡耕地产流产沙及氮磷养分流失特征。结果表明：A型（小雨量、短历时、低雨强）降雨发生频率最高，占降雨样本数的71%，B型（大雨量、长历时、中雨强）和C型（中雨量、中历时、高雨强）降雨是引发土壤侵蚀的主要降雨类型。不同土壤管理措施下侵蚀性降雨的临界降雨量差异不明显，降雨量＞10 mm时坡面开始产流产沙。A型降雨下产流量：CK(123.49 L)＞HA(60.67 L)＞GM(53.67 ...
常松果, 胡雪琴, 史东梅, 丁文斌, 蒋   +4 more
doaj  

[A protein-specific quantitative detection method based on polyacrylamide gel electrophoresis and online fluorescence imaging]. [PDF]

Se Pu
Zou R, Yu ZX, Guo ZH, Dai HZ, Zhang Q, Liu WW, Cao CX.   +6 more
europepmc   +1 more source

[Advances in the application of exhaled breath analysis technology in the detection and diagnosis of liver failure]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Zhang ST, Liu YS, He YL.
europepmc   +1 more source

[Research progress on high performance liquid chromatography stationary phases based on mixed mode]. [PDF]

Se Pu
Li SJ, Li Z, Tang ZX, Lyu M, Wang LT.
europepmc   +1 more source

[Determination of six whitening ingredients in cosmetics by ultra performance liquid chromatography coupled with photodiode array detector and corona charged aerosol detector]. [PDF]

Se Pu
Li X, Wang Y, Fan F, Zhou W, Chen B, Shi H, Cai G, Liu Y, He Y.   +8 more
europepmc   +1 more source

[Research progress in the application of molecular imprinting technology in catalysis]. [PDF]

Se Pu
Ye WM, He DC, Cui XJ, Ma HW, Qian B, Shi F.   +5 more
europepmc   +1 more source

[Antimicrobial resistance characteristics of <i>Escherichia coli</i> in infants younger than 90 days]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Zhang DM, Zhou L, Chen Y, Zou PC, Xiao RQ, Ma LJ, Wang YJ.   +6 more
europepmc   +1 more source

[Plasma lipidomics-based exploration of potential biomarkers of metastasis in pediatric medulloblastoma]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Yang CJ, Xu XQ, Bao L, Wu WS, Jiang DC, Shi ZY.   +5 more
europepmc   +1 more source

[Clinical and molecular characteristics of neonatal <i>Klebsiella</i><i>pneumoniae</i> sepsis]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Hebei Provincial Neonatal Sepsis Research Collaboration Group.
europepmc   +1 more source