人参皂苷Rb1通过抑制NF-κB p65介导的炎症和氧化应 激改善内皮细胞复制性衰老 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2018 【目的】本研究旨在探讨人参皂苷Rb1对内皮细胞复制性衰老的作用和机制。【方法】建立人原代脐 静脉内皮细胞（HUVEC）复制性衰老模型，根据细胞形态的变化、衰老相关β-半乳糖苷酶（SA-β-Gal）染色阳性 率和纤溶酶原激活物抑制剂1（PAI-1）的表达水平评估HUVEC衰老；将复制性衰老模型细胞分为0、20、40、80、 100μmol/LRb1组，不同浓度人参皂苷Rb1处理衰老细胞48h后观察衰老指标SA-β-Gal染色和PAI-1蛋白变 化，每组3个复孔；采用Westernblot方法检测对照组 ...
凌叶盛 +5 more
doaj +2 more sources
人参皂苷Rb1通过Sirt3/SOD2通路延缓高糖诱导的人脐静脉内皮细胞衰老 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2019 【目的】本研究拟探讨人参皂苷Rb1通过调节沉默信息调节因子3/超氧化物歧化酶2（Sirt3/SOD2）通路延缓高糖诱导的人脐静脉内皮细胞（HUVEC）衰老的作用和机制。【方法】建立高糖（40mmol/L葡萄糖）诱导的HUVEC早熟性衰老模型，根据衰老相关β-半乳糖苷酶（SA-β-Gal）阳性率及Ⅰ型纤溶酶原激活物抑制因子（PAI-1）和P16表达评估HUVEC衰老，并采用AnnexinV-FITC/PI排除细胞凋亡的发生。采用Westernblot检测各组Sirt3与SOD2表达的变化 ...
柯世业 +6 more
doaj +2 more sources
人参皂苷Rb1 和Rg1 对模拟移植肺的保护作用 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 1997 摘 要 按照肺移植对供肺获取和保存的方法,将45只家兔的肺分别获取, 保存24 h。然后采用体外装 置,对肺进行通气和灌流,建立模拟肺移植状态的肺缺血再灌注损伤模型。研究人参皂苷Rb1 和Rg1 对肺缺血 再灌注损伤的保护作用及Rb1作用的量效关系。研究结果显示, Rb1 对肺缺血再灌注损伤具有明显保护作用,表 现为使肺组织中超氧化物歧化酶( SOD)含量升高,丙二醛( MDA)含量降低; 使肺动脉压( P PA)降低, 湿肺干肺 比重降低和改善肺组织病理变化。量效关系显示, Rb1 ...
江 沛 孙培吾 麦惠成
doaj +2 more sources
人参皂苷Rb1抗小鼠脑自然衰老及其对mTOR/p70S6K 通路的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2015 【目的】 观察人参皂苷Rb1对小鼠脑组织自然衰老的作用,并探讨mTOR/p70S6K通路在其中的作用?【方法】27只C57/B6雌性小鼠购于中山大学实验动物中心,其中7只3月龄C57/B6雌性小鼠为青年组,20只22月龄C57/B6雌性小鼠为老年组,青年组小鼠予生理盐水(PBS)腹腔注射,老年组小鼠分别予PBS(对照)?低剂量Rb1(10 mg?kg-1?d-1) ?高剂量Rb1(20 mg?kg-1?d-1)腹腔注射?6周后处死小鼠,取脑组织匀浆,检测丙二醛(MDA)含量?单胺氧化酶(MAO)活性 ...
doaj +1 more source
Liver Toxicity of Rare Ginsenosides in Rats after 13 Weeks of Oral Exposure [PDF]
, 2023 Objective: This study aims to evaluate the hepatotoxicity of rare ginsenosides in rats after 90 days of oral administration using heat-transformed rare ginsenosides as the primary material.
Feiyan TAO +6 more
core +1 more source
Optimization of the Best Polar Site for Antioxidant Activity of Panax ginseng Extract Based on Entropy Weight Method and Grey Relational Analysis Combined with Component Analysis [PDF]
Objective: The differences in antioxidant activity of different polar parts of panax ginseng were investigated to screen the best active parts. Method: In this study, the water extract of Panax ginseng was extracted by petroleum ether, chloroform, ethyl ...
Fengqin XIAO +7 more
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Study on the preparation process and quality standard of Ji Shenqi anti-cancer granules [PDF]
Objective To preliminarily screen the preparation process of Ji Shenqi anti-cancer granules (JSQ) and establish the quality standard. Methods The excipient types were examined and the formulation moulding process was preferred using the soft material ...
FU Shujie +8 more
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Alleviating Effect and Mechanism of Ginsenosides on Type 2 Diabetic Liver Lesions [PDF]
Type 2 diabetes is a metabolic disease characterized by chronic hyperglycemia and relative insulin deficiency. As the disease progresses, type 2 diabetes often develop pathological changes in the liver such as inflammatory edema, fatty degeneration and ...
ZHAO Ziqi, ZHANG Qianyi, XU Jiao, GUO Xiaohui
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Optimization of Fermentation Process of Panax quinquefolius L. by Schizophyllum commune and Its Antioxidant Capacity in Vitro [PDF]
In order to improve the economic value of Panax quinquefolius L., this study used Panax quinquefolius L. as raw material to solid state ferment Panax quinquefolius L.
Bo LI +7 more
core +1 more source
Neuropreotective effects and mechanism of regulating glutamate transporters in the mice model of Parkinson's disease [PDF]
, 2017 帕金森病（Parkinson’sdisease，PD）是一种中老年常见的神经系统变性疾病，兴奋性毒性作用是PD发病的关键机制，谷氨酸转运体通过摄取谷氨酸减轻兴奋性毒性效应。研究表明，通过上调谷氨酸转运体的表达，可以减弱兴奋性毒性对神经系统的损伤，从而保护多巴胺神经元，改善PD模型小鼠的运动行为障碍。因此，本课题主要研究谷氨酸转运体在PD发病中的调控策略，探讨通过提高谷氨酸转运体来治疗PD的可能性，分为两部分简述如下。 一、雷帕霉素在PD模型小鼠中上调谷氨酸转运体和IL ...
张云龙
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