ＨＬＡ－Ａ*０２０１转基因小鼠侵袭性肺曲霉病模型的建立与鉴定
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘 要： 【目的】 建立ＨＬＡ－Ａ*０２０１转基因小鼠侵袭性肺曲霉病模型，并进行实验室鉴定。【方法】 在真菌孢子感染前，给予ＨＬＡ－Ａ*０２０１转基因小鼠腹腔注射环磷酰胺。乙醚麻醉后，小鼠经鼻腔吸入３组不同浓度的（３ × １０６、３ × １０７、３ × １０８ ｍＬ－１）烟曲霉孢子悬液。阴性对照组小鼠吸入ＰＢＳ。感染后３６ ｈ，处死各组小鼠，取肺组织病理行ＨＥ、ＰＡＳ以及ＧＭＳ染色分析，以验证感染是否成功并计算肺侵袭性肺烟曲霉感染小鼠模型成模率。肺组织研磨涂皿后行菌落计数，分析小鼠肺烟曲霉负载量。【结果】
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Zhongshan Daxue xuebao. Yixue kexue ban, 2016 摘 要： 【目的】 在宫颈癌Ｈｅｌａ、Ｓｉｈａ细胞中上调ｍｉＲ－１００后，研究细胞侵袭及迁移功能及顺铂耐药性变化并探讨其机制。【方法】 将ｍｉＲ－１００ ｍｉｍｉｃ通过ｌｉｐｏｍａｘ试剂转染至宫颈癌细胞Ｈｅｌａ、Ｓｉｈａ中，细胞主要分为两组：ＮＣ组，为空白对照；ｍｉｒ１００组，为上调ｍｉＲ－１００后的细胞。ｔｒａｎｓｗｅｌｌ法观察细胞侵袭性，划痕实验观察迁移性；ＷＢ测定上皮间质变相关蛋白Ｅ－Ｃａｄｈｅｒｉｎ、Ｓｎａｉｌ、Ｖｉｍｉｎｔｉｎ及ＭＭＰ２、ｕＰＡ的表达；ＣＣＫ８法测定顺铂耐药性。 【结果 ...
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泛素水解酶２２ ｓｉＲＮＡ对宫颈癌ＣＤ１３３＋ 宫颈癌干细胞增殖和侵袭的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2016 摘 要： 【目的】 采用ＵＳＰ２２ ｓｉＲＮＡ对宫颈癌ＣＤ１３３＋ＣａＳｋｉ宫颈癌干细胞进行基因治疗，观察其对ＵＳＰ２２含量和对增殖侵袭能力的影响，并对其分子生物学机制进行初步探讨。【方法】 流式分选获得ＣＤ１３３＋ ＣａＳｋｉ宫颈癌干细胞，分为ＵＳＰ２２组、阴性对照（ＮＣ）组、空白对照组（ＭＯＣＫ）组。采用ｗｅｓｔｅｒｎ ｂｌｏｔ法比较各组细胞ＵＳＰ２２蛋白的含量，ＭＴＴ法检测３组细胞的增殖能力，Ｔｒａｎｓｗｅｌｌ法检测３组细胞的侵袭能力，荧光素酶报告基因实验检测３组细胞Ｗｎｔ／β ...
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[1 case of recurrent nasal vestibular aggressive angiomyxoma]. [PDF]
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[Diagnostic Value of Serological Markers and Quantitative Real-Time PCR in Patients With Invasive Candidiasis]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanMd Ali H, Peng L, Yuan Y, Zhou X, Ma Y.
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[Invasive pulmonary aspergillosis assisted in clinical diagnosis by chest CT pulmonary angiography: three cases report]. [PDF]
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[Aggressive natural killer cell leukemia with hemophagocytic lymphohistiocytosis: a case report]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiYang JH +7 more
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Zhonghua Xue Ye Xue Za ZhiLong KT, Pu ZH, Yang WX.
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