[Application of Hydrashift 2/4 daratumumab assay in eliminating interference of daratumumab on serum immunofixation electrophoresis]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2021 目的 探讨达雷妥尤单抗治疗浆细胞疾病后对免疫固定电泳结果产生的干扰及消除干扰的方法。 方法 收集2020年4月至2021年3月北京大学人民医院应用达雷妥尤单抗治疗的8例浆细胞疾病患者的血清样本，分别进行标准的免疫固定电泳检测及Hydrashift 2/4 daratumumab检测。 结果 达雷妥尤单抗治疗后，81.3％（13/16）的样本出现药物性单克隆条带（IgG-κ型），未出现药物性单克隆条带的样本考虑与个体差异、给药间隔时间、患者免疫球蛋白水平相关。出现IgG-κ型单克隆条带的样本中76.9 ...
Xu S +6 more
europepmc +2 more sources
[A protein-specific quantitative detection method based on polyacrylamide gel electrophoresis and online fluorescence imaging]. [PDF]
Se Pu蛋白质的特异性检测在生物分析和临床诊断中具有重要意义。现有方法如免疫固定电泳和蛋白质印迹法，需要手工染色和脱色等步骤，操作繁琐，耗时久且定量能力有限。本文结合在线荧光成像技术开发了一种蛋白质免疫聚丙烯酰胺凝胶电泳（PAGE）定量检测方法。该方法首先采用荧光标记抗体结合目标蛋白质，使用甲醛交联免疫复合物后进行电泳和在线成像，最终通过计算凝胶电泳图像中游离抗体的荧光信号强度实现定量分析。整个检测流程可在1.5 h内完成。本文以人转铁蛋白（transferrin，TRF）为模式蛋白进行方法学验证 ...
Zou R +6 more
europepmc +2 more sources
Lenalidomideas a Second-line Therapy Achieves Long-term Remission in Elderly Patients with Relapsed/Refractory Multicentric Castleman's Disease：a Report of Two Cases and Literature Review [PDF]
, 2022 No standard therapy is recommended for elderly multicentric Castleman's disease (MCD) patients who failed to the first-line treatment or relapsed. Lenalidomide has been reported to be effective for relapsed/refractory MCD in foreign countries, but ...
TANG Yangming, SHI Renzhou, LAN Mei, LI Xuejun, WEN Zonghua, LU Xiaochen
core +1 more source
Clinical pathway for diagnosis and treatment of new diagnosed multiple myeloma in Guangxi [PDF]
Multiple myeloma, an incurable clonal plasma cell dysplasia, is the second most common hematological malignancy. Standardized diagnosis and treatment are crucial for prolonging the survival of myeloma patients and improving the quality of their lives ...
Guangxi Plasma Cell Disease Collaboration +1 more
core +1 more source
Multiple myeloma with cardiac amyloidosis and secondary adrenal cortical dysfunction: a case report [PDF]
Multiple myeloma is a malignant proliferative disease of plasma cells, and some patients may develop systemic amyloidosis. Cardiac amyloidosis is a common cause of death in these patients.
CHEN Haifei, ZHOU Lingyun
core +1 more source
Research progress of the immunosuppressive tumor microenvironment in multiple myeloma [PDF]
Multiple myeloma (MM) is a hematological malignancy originating from the immune system, and the immune microenvironment plays a pivotal role in its pathogenesis and progression.
Peng LIU, Yue WANG
core +1 more source
Zhongguo shiyan zhenduanxue, 2012 患者,赵某某,男,75岁,于2003年7月因"面色苍白、乏力1个月"就诊,经骨穿示:成熟红细胞成缗钱样排列,原浆细胞+幼浆细胞占29%,免疫球蛋白定量:IgG 4.83g/L、IgA 0.51g/L、IgM 0.4g/L,均低于正常,血清蛋白电泳未见明显M峰,血清免疫固定电泳阴性,尿本周蛋白阴性。血常规:血红蛋白73g/L,血小板:130×109/L;肾功能 ...
侯俊杰 +5 more
doaj
Linchuang shenzangbing zazhi, 2020 病例资料患者,女,48岁,因"间断双下肢水肿4年余,纳差1个月余"入院,患者4年间断出现双下肢水肿,活动后加重,自觉尿中泡沫增多,就诊于当地医院,化验尿蛋白阳性,给予输液(具体不详)对症处理后好转出院,仍反复出现双下肢水肿,多次复查尿潜血阳性,蛋白阴性,未检测肾功能,未予治疗。1个月前因牙痛自行口服氯芬待因2片,出现恶心、呕吐,尿量无明显变化,就诊于当地医院,输液治疗2 d,恶心、呕吐明显缓解,出现乏力、纳差 ...
NING Shao-xiong +7 more
doaj
Zhongguo shiyan zhenduanxue, 2011 目的探讨多发性骨髓瘤（MM）的常见实验室诊断指标对分型、治疗和预后的意义。方法观察40例MM患者骨髓细胞形态及细胞化学染色情况,并进行血清蛋白电泳、免疫固定电泳及血清β2-微球蛋白（β2-MG）、乳酸脱氢酶（LDH）检测。结果 MM患者的骨髓瘤细胞具有较高的异质性,酸性磷酸酶染色（ACP）,酸性α-醋酸萘酚酯酶染色（ANAE）,过典酸-雪夫染色（PAS）对骨髓瘤细胞分型具有鉴别诊断意义;血清蛋白电泳M蛋白检出率为84.0%,59.0%出现在γ区,25.0%出现于β与γ区间,16.0%出现于β区 ...
翟洪顺 +6 more
doaj
Zhongguo shiyan zhenduanxue, 2010 目的回顾分析208例多发性骨髓瘤（MM）病例的骨髓细胞学、临床分型以及血清免疫球蛋白、免疫固定电泳结果 ,探讨其在MM诊断中的作用。方法回顾分析208例MM患者骨髓细胞学特点,按照欧洲血液学会议形态学分型标准将骨髓瘤细胞分型,计数骨髓瘤细胞比例;按临床特点和分期标准将病人分期;记录病人血清免疫球蛋白、免疫固定电泳结果。结果 208例MM病例中:骨髓涂片中骨髓瘤细胞比例≥10%的204例（98.1%）,骨髓瘤细胞比例在6.0%-10.0%的3例（1.4%）,骨髓瘤细胞比例不增高的1例（0.5 ...
马海梅 +3 more
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