Results 131 to 140 of about 17,251 (213)

华支睾吸虫的一个 μ 型 GST 基因的表达、纯化与免疫反应性鉴定

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2004
【目的】扩增华支睾吸虫一个μ型谷胱甘肽移酶(mu-classglutathionetransferaseofClonorchissinen-sis,CsGSTM)基因,明确是否存在内含子,进行原核表达,纯化其表达产物,鉴定免疫反应性。【方法】用PCR方法从成虫cDNA文库和基因组中扩增出目的基因,测序,定向克隆到原核表达载体pET-30a(+),在大肠杆菌BL21/DE3表达,按照Ni-NTAagarose说明书纯化重组蛋白,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodiumdode ...
doaj  

[Research progress on protein lactylation modification in malignant tumors]. [PDF]

open access: yesBeijing Da Xue Xue Bao Yi Xue Ban
Dong H, Chen H, Zhang C.
europepmc   +1 more source

[Novel clinical insights and frontier issues in alpha- fetoprotein-producing gastric cancer]. [PDF]

open access: yesBeijing Da Xue Xue Bao Yi Xue Ban
Luo B, Liu H, Xie W, Gong W.
europepmc   +1 more source

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