Results 111 to 120 of about 16,345 (164)

无动物源性成分人胚胎干细胞培养体系与其它人胚胎干细胞培养体系的比较

Zhongshan Daxue xuebao. Yixue kexue ban, 2012
【目的】 通过比较ｈＥＳＣ在各培养体系中未分化表面标记物及以多能性因子的表达，进一步筛选出更优化、安全有效的ｈＥＳＣ培养体系。【方法】 四种培养体系分别为：新建立的无动物源性的人包皮饲养层（ＸＦ－ＨＦＦ）联合成分确定培养基（ＣＤＭ）的无动物源性培养体系（ＸＦ－ＨＦＦ／ＣＤＭ），对照组为ＸＦ－ＨＦＦ联合添加了２００ ｍＬ／Ｌ人血清（ＨＳ）的培养基的培养体系（ＸＦ－ＨＦＦ／ＨＳ），ＨＳ包被的培养皿联合ＣＤＭ的无饲养层培养体系（ＨＳ－Ｍａｔｒｉｘ／ＣＤＭ），以及传统的人包皮饲养层（ＸＣ－ＨＦＦ ...
doaj  

间充质干细胞及其培养上清与融冻产物对脐血CD34+ 细胞扩增及分化的作用

Zhongshan Daxue xuebao. Yixue kexue ban, 2010
【目的】 探讨人骨髓间充质干细胞(MSC)及其培养上清65380;融冻产物对脐血CD34+ 细胞扩增及分化的作用65377;【方法】 分离纯化人骨髓MSC,收集纯化三代的MSC培养上清,采用反复冻融法获得MSC融冻产物,采用免疫磁珠分选系统纯化脐血CD34+ 细胞65377;实验分为4组,分别为含MSC滋养层组65380;MSC上清组65380;MSC融冻产物组及单纯造血生长因子(HGFs)对照组65377;流式细胞仪检测人脐血CD34+ 细胞在这四种培养体系中第6天和第12天有核细胞数及CD34 ...
doaj  

[Human umbilical cord mesenchymal stem cell grafting alleviates inflammatory response in type 1 diabetic mice by suppressing M1 macrophage polarization through Chi3l1]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Liu X, Xu Y, Sheng H, Liu H.
europepmc   +1 more source

[Cannabinoid receptor 2 inhibition on acantholysis in oral mucosal pemphigus]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Liu H, Song P, Hou Y, Huo X, Mi L, Liu C.   +5 more
europepmc   +1 more source

[A follow-up study on the physical and motor development of premature infants with small for gestational age within 2 years after birth]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Zheng P, Lin K, Liu M, Zhao X, Xiao Y, Xing Y.   +5 more
europepmc   +1 more source

[The role of extracellular vesicles in early embryo development and their application in assisted reproductive technologies]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Wang H, Li X, Lan H, Tong X.
europepmc   +1 more source

神经干细胞的单层培养及分化过程中ＨＥＳ１和ＭＡＳＨ１的表达变化

Zhongshan Daxue xuebao. Yixue kexue ban, 2014
摘 要： 【目的】 建立神经干细胞（ＮＳＣ）体外培养方案，使ＮＳＣ的体外可以得到增殖和分化，并检测ＮＳＣ分化过程中ＨＥＳ１和ＭＡＳＨ１的表达变化。【方法】无菌条件下取ＳＤ大鼠胚胎端脑，无血清悬浮培养，形成Ｐ２代神经球后，将神经球消化成单细胞，分为神经球悬浮培养和单层贴壁培养，并对两种不同培养方式下的ＮＳＣ进行干性鉴定和分化能力鉴定。用不同浓度（２０、４０、８０ μｇ／Ｌ）的脑源性神经生长因子（ＢＤＮＦ）诱导ＮＳＣ分化，选出最合适的浓度。用ＢＤＮＦ最佳诱导浓度诱导ＮＳＣ分化，Ｑ ...
doaj  

[Construction of cardiac organoids derived from human induced pluripotent stem cells for cardiac disease modeling and drug evaluation]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Gong X, Fan Y, Luo K, Yan Y, Li Z.
europepmc   +1 more source

[Angio-associated migratory cell protein is highly expressed in hepatocellular carcinoma and promotes tumor cell invasion and metastasis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Li C, Yin G, Cheng X, Jiang Y.
europepmc   +1 more source

加拿大人工智能发展现状 [PDF]

, 2019
周伯柱, 赵晏强
core