Zhongguo shiyan zhenduanxue, 2006 目的探讨HCMV感染对体外培养内皮细胞粘附分子表达的影响。方法应用双抗体夹心酶联免疫分析法及细胞ELISA方法检测体外培养人脐静脉血管内皮细胞在感染HCMV后,血管内皮细胞粘附因子（VCAM-1）及可溶性细胞间粘附因子1（sICAM-1）表达的变化。结果感染组细胞培养液sICAM-1 24小时和对照组相比明显升高达10.7±2.4μg/L,而正常对照为5.7±3.1μg/L,而后48小时、72小时均高于相应对照组 ...
姚磊 +4 more
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 选择最佳人源饲养层,并检测不同组织来源的人源饲养层细胞碱性成纤维细胞生长因子(bFGF)的分泌情况,探讨其水平与人胚胎干细胞(hESCs)生长是否相关65377; 【方法】 原代培养包皮真皮65380;子宫内膜基质65380;绒毛65380;输卵管65380;胎儿真皮65380;胎儿肌肉及胎鼠成纤维细胞(MEFs);按组织来源分为7组(MEFs为对照),同期接种同一株hESCs,从饲养层细胞密度65380;丝裂霉素作用时间等,寻找不同饲养层适合hESCs生长的最佳条件;按人体组织分6组 ...
doaj
Zhongguo shiyan zhenduanxue, 2006 目的利用原子力显微镜（AFM）原位观察正常新西兰白兔主动脉和主动脉瓣膜内皮细胞表面超微结构。方法取10只正常健康雄性新西兰白兔的新鲜主动脉和主动脉瓣膜组织,经磷酸盐缓冲液去除组织表面杂质后,置于1%的甲醛溶液中固定。在大体显微镜下,将固定好的主动脉和瓣膜组织粘附并展平在粘着有双面胶的载玻片上,并将主动脉的内膜面和主动脉瓣膜的动脉面置于上部,待样本干燥后,将其置于NanoScope IIIa AFM载物台上,选择其中某个区域,在Tapping模式下进行扫描 ...
尹彤 +6 more
doaj
[Astragaloside IV alleviates D-GAL-induced endothelial cell senescence by promoting mitochondrial autophagy via inhibiting the PINK1/Parkin pathway]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoYi M +6 more
europepmc +1 more source
[Indole-3-acetic acid alleviates <i>Cryptococcus neoformans</i>-induced pyroptosis in cerebral microvascular endothelial cells by regulating stress granule-mediated NLRP3 inflammasome activation]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoChen J +5 more
europepmc +1 more source
Trimethylamine oxide induces pyroptosis of vascular endothelial cells through ALDH2/ROS/NLRP3/GSDMD pathway. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2022 Li J +5 more
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2013 【目的】 探讨雌二醇(E2)及雌酮(E1)对体外培养的人子宫内膜腺上皮细胞及间质细胞间隙连接细胞间通讯(GJIC)及连接蛋白(Cx)43?32表达的影响?【方法】 采用划痕染料标记示踪技术及激光共聚焦显微镜扫描技术(LSCM),观察E2及E1对原代培养的20例人子宫内膜腺上皮细胞及间质细胞GJIC的影响,采用激光共聚焦显微镜扫描技术,观察E2及E1对Cx 43?Cx 32表达的影响?【结果】 5 × 10-5 mol/L雌二醇作用48 h后间质细胞及腺上皮细胞GJIC功能没有明显变化(P > 0.05),
doaj
[Berberine ameliorates coronary artery endothelial cell injury in Kawasaki disease through complement and coagulation cascades]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiLiao JW, Guo X, Liang B, Li XX, Xu MG.
europepmc +1 more source
[FGF19 alleviates inflammatory injury in vascular endothelial cells by activating the Nrf2/HO-1 signaling pathway]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiZhang YJ +6 more
europepmc +1 more source
[Mechanism of tetramethylpyrazine attenuates inflammatory injury in endothelial cells by activating the SIRT1 signaling pathway]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiChen LP +5 more
europepmc +1 more source