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转录因子KLF16对非酒精性脂肪肝病脂质代谢的影响 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban目的探究转录因子KLF16在非酒精性脂肪肝病（NAFLD）中的表达改变以及其对脂质代谢的影响。方法通过高脂饮食诱导小鼠构建NAFLD的动物模型。将小鼠分为正常饮食组（ND）和高脂饮食组（HFD）；油酸诱导小鼠肝脏原代细胞构建NAFLD细胞模型。将细胞分为对照组（Control）和油酸诱导组（OA）；实时荧光定量PCR （RT-qPCR）和蛋白免疫印迹技术（Western blot）方法检测NAFLD动物和细胞模型中KLF16的表达变化。通过小鼠尾静脉注射腺相关病毒（AAV ...
蔡冠军 +3 more
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Mechanism of blood-brain barrier damage caused by the inhibition of Wnt7/β-catenin pathway induced by endoplasmic reticulum stress in cerebrovascular endothelial cells after stroke [PDF]
, 2023 Objective·To investigate whether the inactivation of Wnt7/β-catenin signaling causes the destruction of the blood-brain barrier (BBB) in cerebrovascular endothelial cells after ischemic stroke, and investigate whether endoplasmic reticulum stress bursts ...
DONG Haiping +3 more
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全球能源互联网, 2023 “互联网+智慧能源”（即能源互联网）的发展理念，集中体现着数字化技术对能源系统的设计、运行和发展的全局优化。从能源系统热力学熵和信息系统信息熵的理论及视域，阐释能源互联网综合能源系统及其对应能源物联网的熵增与熵减机制。首先，在熵视域下揭示能源互联网所体现出的信息熵与热力学熵之间的内在关联，即通过信息的引入充分激发能量系统的自组织性，减少能源网络的无序程度。同时，以能源互联网范畴中的多能互补综合能源系统和数字孪生能源物联网为对象，以熵理论揭示信息物理融合系统中信息流与能量流的协同作用 ...
孙艳玲 +5 more
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酪氨酸激酶抑制药GW2974诱导乳腺癌细胞BT474耐药的代谢机制研究 [PDF]
, 2019 目的通过比较酪氨酸激酶抑制药GW2974给药后母本细胞株BT474与耐药株r BT474之间代谢相关因子mRNA表达的差异,探讨r BT474可能的耐药机制。方法收集对数生长期r BT474和BT474细胞,接种于96孔板,加入含有GW2974的培养基,分别于孵育0,12,24和48 h后,通过噻唑蓝法检测BT474与r BT474细胞的增殖情况。用逆转录实时定量-聚合酶链反应检测12种相关代谢因子葡萄糖转运体4(GLUT4)、2,6-二磷酸果糖激酶(PFK-2)、丙酮酸激酶2(PKM2)、乳酸脱氢酶 ...
吴光华 +6 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2023 目的探究磷酸二酯酶5（PDE5）抑制剂西地那非（SIL）或LW1646对单侧输尿管结扎（UUO）诱导的肾间质纤维化的保护作用。方法C57BL/6雄性小鼠被随机分为假手术Sham（n=6）、7UUO（n=6）、7UUO+SIL（n=6）或7UUO+LW1646（n=6）组，术前1h给予SIL或LW1646、或溶剂灌胃给药，连续给药7 d后处死小鼠，收集梗阻侧肾脏标本。对肾组织进行H&E和Masson’s染色，使用免疫印迹和RT-qPCR检测肾脏中的纤维化、内质网应激、自噬 ...
翟庆娴 +4 more
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Renal protective effect and mechanism research of hypoxia inducible factor-1α inhibitor YC-1 in diabetic nephropathy mice [PDF]
, 2023 Objective·To investigate the effect of hypoxia inducible factor-1α (HIF-1α) inhibitor YC-1 on the progression of diabetic nephropathy (DN) in mice and the potential mechanism.Methods·Ten-week-old male db/db mice (DN model) and their nondiabetic wild-type
FAN Ying +5 more
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Research progress on the association between melatonin and hypertensive disorder complicating pregnancy [PDF]
, 2023 Melatonin (N-acetyl-5-methoxytryptamine) is a polypotent neuroendocrine lipid-soluble small molecule secreted mainly by the pineal gland. During gestation, melatonin levels in the mother at night rise as the pregnancy progresses and return to normal ...
KANG Wenhui +4 more
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Function of Apoptosis and Autophagy in Cardiovascular Diseases [PDF]
, 2017 细胞凋亡与自噬是普遍存在于各种细胞内的生命现象,广泛参与了机体的生理、病理过程,且二者有着多因子、多通路的相互影响,这种错综复杂的关系贯穿于各种心血管疾病的始终,对多种心血管疾病的发生发展转归起重要作用。现对细胞凋亡与自噬及其相互关系进行梳理,并综述其在心血管疾病中的作用。Apoptosis and autophagy are common life phenomenon in various cells, which widely involve in physiological and ...
杨雅瑾, 林小凤, 费燕, 陈尚瑜
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Research progress of subcellular structure-targeted therapy in spinal cord injury [PDF]
, 2023 Spinal cord injury is a serious disease that greatly affects the sensory function, motor function and autonomic nervous function of patients. It not only brings serious physical and mental harm to patients but also causes huge economic burden to the ...
LIN Junqing, LIU Tiexin, ZHENG Xianyou
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丹参素对缺氧/复氧诱导H9C2心肌细胞损伤和内质网应激的改善作用
Zhongguo shiyan zhenduanxue, 2020 目的观察丹参素(DLA)通过抑制内质网应激和凋亡对H9C2心肌细胞缺氧/复氧(H/R)后损伤的保护作用。方法将离体培养的H9C2心肌细胞随机分为3组:对照组、缺氧/复氧组(H/R组)、DLA+H/R组(DLA组)。H/R组先缺氧6h后复氧24h;DLA组于缺氧时给予DLA(10-6M)培养6h,再复氧24h。对照组心肌细胞正常培养至实验结束。ELISA测定细胞中乳酸脱氢酶(LDH)、肌酸激酶MB同工酶(CK-MB)和丙二醛(MDA)的含量;Western ...
丁玉红, 郭秀颖
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