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Research advances Progress in mechanistic studies of renal fibrosis based upon regulatory cell death [PDF]
As a basic pathological feature at advanced stage of various kidney diseases, renal fibrosis (RF) is an important cause of kidney failure. It is characterized by an excessive accumulation of extracellular matrix (ECM), damage of renal tissue structure ...
Guang-ming Yin +5 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2023 目的构建稳定共表达荧光素酶（Luc）和增强型绿色荧光蛋白（EGFP）的人源卵巢癌细胞SKOV3（SK-Luc-EGFP）并探究其在卵巢癌腹腔转移的体内外研究中的应用。方法利用重叠PCR扩增得到的Luc-T2A-EGFP基因片段，构建重组质粒pCDH-Luc-T2A-EGFP-Puro；采用三质粒慢病毒包装系统转染HEK 293T细胞，收集病毒液感染SKOV3细胞；通过嘌呤霉素筛选，流式细胞术检测，筛选高效表达EGFP的细胞（SK-Luc-EGFP），并通过体外生物发光实验验证Luc的表达。对SK-Luc-
万里舟, 杜汝沛, 陈康梅
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Role of selenoprotein M knockdown in the melatonin antagonism of nickel-induced apoptosis and endoplasmic reticulum stress in mouse heart. [PDF]
J Zhejiang Univ Sci B, 2023 Zhang X +8 more
europepmc +1 more source
Zhongguo shiyan zhenduanxue, 2014 目的探讨内质网应激在糖尿病心肌病的作用及人参果皂苷的干预作用及其机制。方法将50只Wistar大鼠随机分为正常对照组（C组）10只;另40只予高脂高糖饲养4周后腹腔注射链脲佐菌素（STZ）40mg/kg×4周,成功制备糖尿病大鼠模型25只,再随机分为糖尿病模型组（D组,n=10）及人参皂苷给药组（G组,n=15,40mg/kg/d给药12周）,处死大鼠,取血测定空腹血糖、总胆固醇（TC）、甘油三酯（TG）、和心肌酶水平。留取心脏组织观察心脏形态改变,Tunel法检测心肌细胞凋亡 ...
赵良辰, 真娟, 尉海涛
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Zhongguo shiyan zhenduanxue, 2022 ＜正＞放射治疗是肺癌患者的主要治疗手段之一，可以提高肿瘤的局部控制率。但由于肺是射线敏感器官，其组织细胞容易受到辐射的损坏[1]从而可以引发放射性肺损伤（RILI）。RILI的发病机制可能与炎症反应、肺泡及内皮细胞损伤等有关[2]。在体外研究中，细胞外组蛋白可损伤内皮细胞[3]、刺激细胞因子的释放[4]，并可以诱导嗜中性粒细胞细胞外诱捕网的形成和髓过氧化物酶的释放。因此，推测细胞外组蛋白可能作为促炎介质在RILI的发生发展中占据重要地位。本研究拟通过收集接受胸部放疗的肺癌患者并利用细胞实验 ...
刘立杰 +9 more
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[A Review of the Roles of Endoplasmic Reticulum Stress in Cancer Cell Metastasis]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue Ban, 2021 Zhou JF, Zhou Q, Chen C, Pan JX.
europepmc +1 more source
Research progress in the role of β-arrestin 1 in the regulation of inflammatory response [PDF]
The inflammatory response is a systemic process produced by the body in response to stimulation such as infection or injury. The inflammatory response is closely related to the pathogenesis of various diseases, and plays an important role in the ...
FAN Yixuan +4 more
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Linchuang shenzangbing zazhi, 2017 急性肾损伤（acute kidney injury,AKI）是一种造成多器官损伤的临床危重症,尽管AKI相关的基础研究及临床治疗在不断进步,但仍然无法解决AKI住院患者住院时间长、耗费医疗资源多、生存质量和预后差、加重家庭和社会经济负担等诸多问题[1-2]。目前研究发现,肾小管上皮细胞凋亡坏死、氧化应激、炎症、内质网（endoplasmic reticulum,ER）的功能紊乱等在AKI发病机制中起重要作用。
刘杰, 朱凯, 杨定平
doaj
Endoplasmic Reticulum Stress in RGC lesions in DR: Protective Role of an aqueous extract of Radix Astragali， Radix Angelica Sinensis and Panax Notoginseng [PDF]
, 2016 目的：早期糖尿病视网膜病变包括神经病变与血管病变，且神经病变可能早于并加剧血管病变的发生，然而现有的治疗方式大多靶向血管病变且疗效有限。我们既往研究表明当归补血汤加味方(RRP)可有效缓解早期糖尿病视网膜的血管病变，也能减少视网膜内神经节细胞的凋亡。已知ERstress广泛存在于应激的真核细胞内，并在糖尿病视网膜病变所致炎症和细胞凋亡中发挥重要作用。本研究拟探索当归补血汤加味方(RRP)对糖尿病视网膜神经节细胞(RGC)内质网应激的作用及其与神经节损伤的关系及其调控机制。 材料与方法 ...
卢述华
core
Oxamate alleviates silicotic fibrosis in mice by inhibiting senescence of alveolar type II epithelial cells [PDF]
BackgroundThe senescence of alveolar type II epithelial cells is an important driving factor for the progression of silicotic fibrosis, and the regulatory effects of oxamate on the senescence of alveolar type II epithelial cells is still unclear ...
Fuyu JIN +7 more
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