Results 31 to 40 of about 3,135 (166)
Zhongguo shiyan zhenduanxue, 2014 目的评价基因芯片技术在结核病防治工作中对结核分枝杆菌异烟肼和利福平耐药性的检测效果。方法利用基因芯片技术对38例涂阳肺结核患者的痰标本进行异烟肼和利福平耐药检测,以传统罗氏药敏试验为金标准,对基因芯片技术的检测效果进行评价。结果在38例菌株中,用基因芯片法进行异烟肼耐药基因检测,与罗氏药敏的符合率为84.2%,对38例进行利福平耐药基因检测,符合率为89.5%。结论基因芯片检测异烟肼和利福平的耐药性与罗氏药敏方法具有很好的一致性,具有简便快速,灵敏度高,特异性好的优点 ...
孙炳奇 +4 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 2023 目的建立利福平耐药基因rpoB高通量突变文库。方法通过同源重组和L5 attB噬菌体整合位点交换构建耻垢分枝杆菌ΔrpoB attB：：rpoB菌株；基于L5 attB质粒交换系统和抗性选择培养基，挑取48个克隆以验证质粒替换的效率；针对利福平耐药决定区（RRDR）区域内以每３个碱基为单位设计简并性引物，通过PCR扩增获得该区域81个碱基的全覆盖式突变文库；文库片段与载体进行无缝克隆转化到大肠杆菌中形成大肠杆菌突变文库；基于质粒交换原理，将突变质粒库转化至耻垢分枝杆菌ΔrpoB attB ...
陈勇, 冯思源, 徐霖, 田国宝
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Whether therapeutic drug monitoring is necessary for linezolid? [PDF]
, 2016 利奈唑胺非肝药酶代谢,约35%以原型经肾排泄,说明书提示其浓度受其他药物影响轻微,肾功能不全患者无需调整剂量,但临床研究表明肾功能不全患者利奈唑胺所致血小板减少症或贫血发生率增高与利奈唑胺高暴露相关,且利奈唑胺与某些药物存在的相互作用可显著影响其血药浓度、利奈唑胺的血药浓度在危重症患者中个体差异大,应进行血药浓度监测。Linezolid is not metabolized by CYP450 s and only approximately 35% of the prototype was ...
余剑华, 卢志品, 曹伟
core
Child Rights Impact Assessment: A Policy Tool for a Rights‐Respecting Digital Environment
Policy &Internet, Volume 17, Issue 3, September 2025.ABSTRACT Businesses that develop digital products and services often anticipate generic users, paying insufficient attention to the needs and rights of children who may use or be impacted by their practices. This article investigates the potential of child rights impact assessment (CRIA) as a policy tool to protect and respect children's rights in the ...
Sonia Livingstone, Kruakae Pothong
wiley +1 more source
Multicolor Melting Curve Analysis-based on Detection of Pyrazinamide-Resistant Mycobacterium tuberculosis [PDF]
, 2017 结核病是由结核分枝杆菌感染人体的慢性传染性疾病。2015年新增患病人数高达1040万，有140万人死于结核病。在众多治疗结核病药物中，吡嗪酰胺是较早发现的一线抗结核药物之一。在酸性环境下，吡嗪酰胺可以抑制巨噬细胞内半休眠结核分枝杆菌。随着吡嗪酰胺广泛使用，耐受吡嗪酰胺的结核病危害面广，因此吡嗪酰胺耐药突变检测的研究急需开展。针对上述问题，本文利用探针熔解曲线分析技术对结核分枝杆菌吡嗪酰胺耐药突变检测展开研究。 第一章绪论，首先简要概述现今世界结核病概况，再介绍耐药结核病诊断和治疗的方法 ...
李梓
core
Conservation benefits of a large marine protected area network that spans multiple ecosystems
Conservation Biology, Volume 39, Issue 4, August 2025.Abstract Marine protected areas (MPAs) are widely implemented tools for long‐term ocean conservation and resource management. Assessments of MPA performance have largely focused on specific ecosystems individually and have rarely evaluated performance across multiple ecosystems either in an individual MPA or across an MPA network.
Joshua G. Smith +23 more
wiley +1 more source
Policy Studies Journal, Volume 53, Issue 3, Page 681-700, August 2025.Abstract OECD countries are grappling with a policy design dilemma, as they strive to balance fiscal sustainability with the increasing demands on the welfare state. This study introduces a new perspective on policy design that integrates the two elements of “social cohesion,” comprising government trust and civic engagement.
Jiwon Kim
wiley +1 more source
Huli yanjiu, 1997 利福平致过敏反应１例ＡＣａｓｅｏｆＡｌｅｒｇｉｃＲｅａｃｔｉｏｎＣａｕｓｅｄｂｙＲｉｆａｍｐｉｎ潘月芳丁胜霞①ＰａｎＹｕｅｆａｎｇ，ＤｉｎｇＳｈｅｎｇｘｉａ(ＦｉｒｓｔＰｅｏｐｌｅ’ｓＨｏｓｐｉｔａｌｏｆＤｅｑｉｎｇＣｏｕｎｔｙ，Ｚｈｅｊｉａｎｇ３１...
潘月芳, 丁胜霞
doaj
Influences on cannabis policy design and adaptation in the American states
Policy Studies Journal, Volume 53, Issue 3, Page 723-746, August 2025.Abstract Despite federal prohibitions, three‐quarters of states had implemented medical marijuana laws (MML) and one‐third had implemented recreational cannabis laws (RCL) by 2020. State policy designs varied considerably, and we focus on policy development and maturation in policy implementation, examining factors influencing design features of policy
Grant W. Neeley +1 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2013 目的利用结核分支杆菌噬菌体裂解法检测结核杆菌对利福平、异烟肼、链霉素敏感性。方法选用传统方法判定为利福平、异烟肼、链霉素的耐药或敏感菌株,采用噬菌体裂解法检测其对以上三种药物的敏感性,对比其特异性和敏感度。结果运用噬菌体裂解法检测结果显示,异烟肼药物浓度为1μg/ml时,其灵敏度为87.0%,特异度为93.3%,与传统方法的符合率为92.0%;链霉素药物浓度为4μg/ml时,其灵敏度为96.2%,特异度为92.0%,与传统方法的符合率为92.9%;利福平药物浓度为5μg/ml时,其灵敏度为91.4 ...
于宏波, 宋阳, 齐红, 韩忠波
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