Zhongshan Daxue xuebao. Yixue kexue ban, 2004 【目的】构建前列腺癌患者的噬菌体抗体库,为前列腺癌特异性的人源性抗体Fab片段的筛选及其应用奠定基础。【方法】由前列腺癌患者外周血分离得淋巴细胞,完整提取其总RNA,经RT-PCR及半套式扩增得到免疫球蛋白全部轻链和重链Fd段基因,然后经酶切、纯化、连接等步骤克隆进载体pComb3,并电转化大肠杆菌XL1-Blue,从而构建前列腺癌患者噬菌体抗体库。【结果】成功构建噬菌体抗体Fab片段库,其轻链及重链Fd段与载体DNA的重组率分别为87%、79%,库容为23.2×107,滴度为87×1012mL-1 ...
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Ki-67、C-erb-B2及nm23在前列腺癌中的表达及意义
Zhongguo shiyan zhenduanxue, 2010 目的探讨相关癌基因等在前列腺癌中表达的意义,从病理学角度了解前列腺癌的生物学特性。方法根据最新WHO分类标准（2002）及Gleason分级法进行组织学分级和对比性分析;应用免疫组织化学技术,观察增殖细胞核抗原Ki-67、原癌基因c-erbB-2、抑癌基因nm23在前列腺癌中的表达情况。结果近81.69%的患者在Gleason分级8分以上;Ki-67与组织学分级之间有显著性差异 ...
毕丽荣, 杨华, 刘仲祥, 王连有
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