Zhongshan Daxue xuebao. Yixue kexue ban, 2012 【目的】 探讨基因干扰Sirtuin3（SIRT3）后对缺氧预处理的影响。【方法】 使用空白载体pGCSIL-GFP进行预实验，筛选适合的感染复数（MOI）。正式实验使用pGCSIL-PUR-SIRT3转染PC12细胞。成功构建空白载体（empty vector）及SIRT3-/-稳定细胞株后，将两种细胞分别分为对照组（control）；缺氧预处理+缺氧组(Hyp+OGD)；单纯缺氧组(OGD)；MTT测定细胞活性。ATP荧光检测试剂盒测定胞内的ATP水平。Western ...
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Seepage-stress-damage coupling analysis of concrete lining under high internal pressure
工程科学与技术混凝土衬砌在水工隧洞中得到了大量使用，但在高内水压作用下其必然开裂引发内水外渗，准确预测衬砌开裂渗漏行为并有效控制，是工程设计上十分关心的问题。本文利用软件ABAQUS内置变量输出和转化，提出简化的渗流体力荷载确定方法；改进衬砌混凝土渗透系数随损伤演化模型，实现不同衬砌混凝土：围岩渗透系数比情况下内水外渗模拟；构建透水薄层单元表征衬砌-围岩交界面，描述衬砌-围岩张开脱离及空腔充水变化过程；在此基础上，实现了基于间接耦合求解的混凝土衬砌渗流-应力-损伤耦合分析 ...
JIN Junchao +4 more
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Jianzhu Gangjiegou Jinzhan为研究标准化Z字形钢结构梁柱连接节点的转动刚度，对翼缘焊接和搭接两种形式的Z字形钢结构梁柱连接节点进行了单调和往复循环加载试验及有限元分析。研究了不同连接节点设计强度对Z字形钢结构梁柱连接节点转动刚度和循环刚度退化等力学性能的影响。结果表明，翼缘焊接和搭接两种形式的Z字形钢结构梁柱连接节点均具有良好的延性和滞回性能。翼缘焊接Z形节点转动刚度表现为刚性连接，翼缘搭接Z字形钢结构节点转动刚度表现为铰接。对于翼缘焊接Z字形钢结构梁柱连接节点，其转动刚度和循环刚度退化程度受焊缝连接强度影响显著 ...
曾立静 +5 more
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[Ultrasound-synergized targeted nanoparticles suppress proliferation, migration and invasion of hypoxic lung cancer cells <i>in vitro</i>]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoDong J, Guo J, Li D, DU Y.
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[ZEB2 promotes glioblastoma progression by reprogramming lipid metabolism through the CYLD/FASN axis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoChen Z, Chai P, Mao Y, Liu R, Song Y.
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[Overexpressing NDUFA13 and CRIP1 improves B cell injury and lung Lesions in COPD mouse models]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoWang M +6 more
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[Preparation of mesoporous covalent organic framework core-shell stationary phase and its application in the analysis of astragaloside Ⅳ content in <i>Astragalus</i>]. [PDF]
Se PuZhao XY +7 more
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[Effect of LncRNA DANCR on the immune microenvironment of glioma cells by regulating the miR-656/BMPR1A axis]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanWang O, Zhu P, Lin J, Wu H.
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[Targeted inhibition of miR-503 upregulates Apelin expression to alleviate myocardial infarction in mice]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi J +10 more
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[Chronic iron overload induces diminished ovarian reserve in mice <i>via</i> iron metabolism dysregulation-mediated oxidative stress and endocrine dysfunction]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoYu J, Chu J, Liang C.
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