Results 171 to 180 of about 29,153 (221)
[Research progress of hydrogel-based growth factors for treatment of intervertebral disc degeneration]. [PDF]
Huo X, Li Z.
europepmc +1 more source
应用基质辅助激光解吸电离飞行时间质谱技术检测载脂蛋白 E 基因多态性
【目的】应用基质辅助激光解吸电离飞行时间质谱技术检测人类载脂蛋白E(apoE)基因的多态性。【方法】用PCR扩增出含apoE两个单核苷酸多态性(SNPs)位点(334T/C和472C/T)的DNA片段作为模板,经延伸反应,产物纯化后利用MALDI-TOFMS进行检测。从而确定样本apoE基因型。【结果】应用本技术对50例广东地区无关个体进行apoE基因多态性检测,共检出3种常见的apoE基因型,其中以apoEΣ3/3型最多,占总数的74.00%;等位基因频率分布以ε3(87.00%)最高。【结论 ...
doaj
[<i>Lactobacillus plantarum</i> ZG03 alleviates oxidative stress <i>via</i> its metabolites short-chain fatty acids]. [PDF]
Lin S +8 more
europepmc +1 more source
[Metabolic reprogramming by glutathione S-transferase enhances environmental adaptation of <i>Streptococcus mutans</i>]. [PDF]
Zheng H, Peng X, Zou J.
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从两例萄糖一6一磷酸脱氢酶变异型——台湾客家型[Gd(-)Taiwan-Hakka]患者基因组DNA中,回收经EcORⅠ完全酶解的3~5 kb片段,与载体λgt10插入重组,用SMR10单菌种系统制备包装提取物,对重组的λgt10进行体外包装,构建成两个基因组基因文库。用32P中标记的G6 PDcDNA成功地从文库中初选出12个阳性克隆,挑选其中4个阳性克隆再次杂交予以证实,提取其中2个克隆DNA,经限制酸酶切电泳及斑点杂交鉴定,分离到该变异型基因的插入片段,并将其克隆到M13 mP19噬菌体,完成Gd(
doaj
[Prediction of protein Kbhb sites based on learnable feature embedding]. [PDF]
Wei Z, Wang Z, Yu J, Deng C, Yu D.
europepmc +1 more source
Physiological analysis of fruit ripening mutants and their application in breeding for long shelf-life [PDF]
Lu, C, Xu, H, Yang, R, Yu, W
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