Detection of 5 kinds of common foodborne pathogens by GeXP multiplex polymerase chain reaction [PDF]
, 2020 Objective To establish a new and rapid GeXP (GenomeLabTM eXpress Profiling) based multiplex polymerase chain reaction (PCR) assay for the detection of five common foodborne pathogens. Methods Nucleotide sequences of specific gene (invA, rfbE, PfrA, IpaH,
Jia REN +3 more
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Zhongguo shiyan zhenduanxue, 2023 目的 探讨大肠埃希菌临床株QD24对黏菌素和碳青霉烯类耐药的机制。方法 通过二代Illumina和三代Nanopore测序技术平台获得大肠埃希菌QD24株的染色体和质粒序列，然后对其进行在线软件分析。液相接合试验、S1核酸酶切脉冲场凝胶电泳(S1-PFGE)和脉冲场凝胶电泳(PFGE))检测菌株携带的质粒。结果 测序分析显示大肠埃希菌QD24属于ST69型，携带了黏菌素耐药基因mcr-1.1和碳青霉烯类耐药基因blaNDM-13,且这两个基因分别位于IncHI2型质粒pQD24-1和IncB/O/K ...
王晓慧 +4 more
doaj
Study on multidrug resistance related genes of Salmonella isolated from food and clinical samples [PDF]
, 2023 ObjectiveTo investigate distribution of integron and extended-spectrum β-lactamase produced by Salmonella isolated from food and clinical samples， and explore the relationship between different drug resistance genes and multidrug resistance ...
GENG Yingzhi +3 more
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Effects of Escherichia coli outer membrane vesicles on proliferation of breast cancer cells and tumor growth of tumor-bearing mice [PDF]
, 2023 Objective·To analyze the effect of Escherichia coli outer membrane vesicle (E.coli-OMV) on the proliferation of 4T1 breast cancer cells in vitro and the inhibition of tumor growth in BALB/c-4T1 tumor-bearing mice in vivo.Methods·OMVs were collected from ...
BAI Jin +5 more
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Investigation and analysis of an outbreak of foodborne disease caused by Salmonella enteritidis in a district of Chongqing [PDF]
, 2023 ObjectiveTo provide the basis for dealing with similar incidents in the future， an outbreak of foodborne illness caused by the consumption of bread contaminated with Salmonella enteritidis was investigated.MethodsDescriptive epidemiological methods were ...
LUO Yu, XIAO Lun, XIONG Fei, YU Jin
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Research progress on microbial contamination and its prevention and control measures in ready-made beverages [PDF]
, 2023 Freshly squeezed fruit and vegetable juice， freshly ground coffee， new tea and other freshly made beverages are very popular among consumers. However， due to the loose quality control of raw materials and non-standard processing operation， the risk of ...
PENG Zixin +4 more
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Analysis of foodborne diseases pathogen infection characteristics in Haining City, Zhejiang Province from 2014 to 2023 [PDF]
ObjectiveTo analyze the epidemiological characteristics of pathogens in foodborne disease cases in Haining City, Zhejiang Province, and to provide a basis for the prevention and investigation of foodborne diseases and outbreak events.MethodsInformation ...
LI Minchao +4 more
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, 2019 活性氧簇(ROS)是生物在有氧环境中进行能量代谢时产生的一类分子的总称,ROS不仅在动物、植物以及细菌的生理过程中发挥着重要的作用,也在研究抗生素杀菌和细菌耐药性的产生上有着重要的功能,添加ROS清除剂有助于我们更好的研究ROS在细菌对抗菌剂耐受中的作用。本文主要通过对常见的ROS清除剂过氧化氢酶、硫脲、联吡啶、DMSO、褪黑素和其他较为常见的清除剂等化合物在抗生素杀菌过程中的作用机制、ROS清除剂添加对细菌耐受性的影响及其他生理作用进行综述 ...
刘小东 +4 more
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Preparation and target protease identification of a cyanobacterial serine protease inhibitor, arthropin [PDF]
, 2023 Objective·To prepare a high-purity cyanobacterial serine protease inhibitor, screen its target proteases, and detect its inhibitory activity.Methods·A novel serine protease inhibitor from Arthrospira platensis was identified in the Expanded Human Oral ...
XU Jiawei, YANG Yufeng, ZHOU Aiwu
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, 2019 细菌外膜囊泡(outer membrane vesicles, OMVs)是一种在不破坏细胞膜的情况下细菌正常分泌的球状小泡。近年来越来越多的研究表明外膜囊泡在病原微生物感染及致病过程中发挥着重要的作用,其作用机...国家自然基金(No.81473251; No.81301474; No.31741006);; 福建省中青年教师教育科研项目资助(No.JT180009);; 厦门大学校长基金(No ...
吴疆, 王岱, 程谦
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