Zhongguo shiyan zhenduanxue, 2009 目的研制高效表达型T载体用于基因的表达及蛋白功能分析。方法限制性内切酶EcoR V酶切真核表达载体pcDNA3,回收线性化质粒片段与三磷酸胸腺嘧啶脱氧核苷酸（dTTP）70℃退火,构成T克隆载体并回收纯化。将增强绿色荧光蛋白基因（EGFP）和中国旱獭α干扰素基因（cwIFNA5）分别扩增后直接克隆到新构建的表达型T载体,转化感受态细菌,挑选阳性菌并制备质粒瞬时转染真核细胞,荧光显微镜观察EGFP的表达,病毒保护试验检测中国旱獭α干扰素的生物学活性。结果成功构建了表达型T载体 ...
卢银平, 祝建芳, 刘朝, 董继华
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】以人包皮成纤维细胞（HFF）为模型,通过系统比较针对不同靶点的小干扰RNA（siRNA）对酸性鞘磷脂酶1（SMPD1）基因的沉默效果,筛选获得最有效的小干扰RNA序列,同时观察沉默SMPD1对细胞凋亡的保护作用。【方法】设计合成三对靶向SMPD1基因的小干扰RNA作为实验组,同时设立阴性对照组和脂质体组（lipofectamine 2000）,瞬时转染原代培养的HFF细胞,采用荧光定量RT-PCR法及Western blot测定SMPD1表达抑制情况,并用化疗药物丝裂霉素诱导细胞凋亡 ...
高军, 周灿权, 张仁礼, 马芸
doaj
Dianci bileiqi, 2013 为改善变电站微机装置运行环境,降低因雷击过电压干扰造成低压弱电设备的损坏程度。介绍了雷电波侵入微机电源系统的途径,分析了电源防雷滤波器的保护原理及其参数的选取原则,考虑成本和容量因素对参数进行了优化设计,确定了电源防雷滤波器的主电路结构及元件参数,并采用电力系统常用电磁暂态仿真软件ATPDraw对相应滤波电路进行了仿真,结果与实验室试验一致,从而证明该防雷滤波器能够有效地抑制雷电引起的电源干扰,保护后续电子设备的安全运行,可为抑制变电站微机电源干扰提供参考。
陈洁 +4 more
doaj
[Regulatory mechanism of interferon regulatory factor 1 by α-synuclein in mouse Parkinson's disease model]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2021 Mu F +5 more
europepmc +1 more source
[Treatment with interferon α-1b, interleukin-2, and thalidomide for persistent RUNX1::RUNX1T1 in a patient with KIT-mutated acute myeloid leukemia after allogeneic hematopoietic stem cell transplantation: a case report and literature review]. [PDF]
Zhonghua Xue Ye Xue Za ZhiMi RH +5 more
europepmc +1 more source
[Interferon-λ1 improves glucocorticoid resistance caused by respiratory syncytial virus by regulating the p38 mitogen-activated protein kinase signaling pathway]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiPeng L +6 more
europepmc +1 more source
[Correlation analysis of cell-free DNA in gingival crevicular fluid with periodontal clinical indicators and cyclic guanosine phosphate-adenosine phosphate synthase-stimulator of interferon genes signaling pathway]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiChen L, Zhu X, Zhou J, Li J, Zhao L.
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2004 【目的】对可能影响严重急性呼吸综合征(SARS)临床病程的多种因素进行Logistic回归分析,初步评价何种因素对SARS病程有影响。【方法】入选的SARS病例共85例(医务人员占92.9%),男15例,女70例,平均年龄(28.9±9.8)岁。以反映临床病程的指标作为因变量,包括持续发热时间,肺部病变初始出现至发展到最高峰的时间,肺部病变吸收时间。以可能影响临床病程的指标作为协变量,包括年龄、性别、潜伏期、初始体温、最高体温、持续发热≥39℃时间、病程中最大受累肺野数目、外周血白细胞减少、抗生素 ...
doaj
[Single-cell RNA sequencing of B cells reveals molecular typing in Sjögren syndrome]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanLin W +6 more
europepmc +1 more source
[Simultaneous screening of pesticides and mycotoxins in dried goji berries and mulberries by QuEChERS-liquid chromatography-quadrupole-time of flight mass spectrometry]. [PDF]
Se PuYin H +7 more
europepmc +1 more source