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愈伤组织 - Open Access .click
Results 1 to 10 of about 4,947 (102)

Anther culture and highly frequent and rapid establishment of embryogenic cell suspensions from Hordeum vulgare L(大麦花药培养及其胚性悬浮细胞系的高频快速建立)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2002
研究了(1)在低温预处理过程中,不同相对湿度对大麦花药出愈率的影响;(2)低温处理愈伤组织对植株分化的影响;(3)高频快速建立大麦胚性悬浮细胞系.结果表明,低温预处理的较大相对湿度能明显促进花药的出愈率;低温处理愈伤组织能提高绿苗率;以7周龄的花药胚性愈伤组织为起始材料高频快速建立了均质、分散性好的胚性悬浮细胞系,建立频率约为25%~50%;已8~9周龄的花药胚性愈伤组织作为起始材料,难以建立胚性悬浮细胞系.
PANJian-wei(潘建伟)   +3 more
doaj   +1 more source

Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2013
以天目铁木嫩茎尖为外植体,应用均匀设计法筛选其基部愈伤组织诱导和愈伤组织再分化芽苗的最适合培养基.结果表明,最适合的嫩茎尖基部愈伤组织诱导培养基为1/2DR+TDZ 2.30 mg • L-1 +2,4-D 0.55 mg• L-1,诱导率为93.5%;愈伤组织芽苗再分化培养基为1/2DR+TDZ 3.30 mg • L-1+KT 0.70 mg • L-1,分化率达99.8%以上.成功建立了天目铁木嫩茎尖离体诱导愈伤组织和芽苗再分化体系,且再生芽苗与野生植株染色体数目相同.
GUDi-zhou(顾地周)   +3 more
doaj   +1 more source

Studies on the high frequency plant regeneration from indica rice immature embryos and its transformation using biolistic method(籼稻幼胚组织培养高频率植株再生及其基因转化的研究)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2000
以籼稻品种特青的幼胚作为外植体,研究了激素对愈伤组织诱导及植株再生的影响。MS+2,4-D 2 mg/L作培养基,愈伤组织诱导率达100%;愈伤组织在MS + KT 3 mg/L + NAA 0.5 mg/L的培养基上,分化频率为67.3%;在此基础上利用基因枪转化技术,将外源的bar基因导入了水稻基因组,转基因植株表现出对除草剂Basta很强的抗性。
WANGHui-zhong(王慧中)   +4 more
doaj   +1 more source

Uniform design for in vitro culture and Plant regeneration through Petals of Fragaria orientalis Lozinsk var. concolor (Kitag.) Liou et C. Y. Li(基于均匀设计优化深山草莓花瓣离体培养及植株再生体系)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2010
应用均匀设计法对深山草莓花瓣诱导愈伤组织和愈伤组织再分化芽苗的主要因子和水平进行了探讨.结果表明,深山草莓花瓣愈伤组织诱导的适宜培养基为LS+TDZ 2.06 mg • L-1+2, 4-D 0.60 mg • L-1,诱导率为98%;愈伤组织再分化芽苗的适宜培养基为1/2 LS + TDZ 2.30 mg • L-1 + NAA 0.10 mg • L-1 + KT 1.30 mg • L-1,分化率为96.3%.炼苗移栽后观察植株的形态、花、果及生长等特征,以筛选具有优良性状的株系.
GUDi-zhou(顾地周)   +6 more
doaj   +1 more source

Studies of cryopreservation of callus of Citrus suavissima Hort. et Tanaka by vitrification(瓯柑愈伤组织的玻璃化法超低温保存研究)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2004
采用玻璃化法对瓯柑愈伤组织进行超低温保存研究.瓯柑愈伤组织在含5%二甲亚砜(DMSO)的MS培养基上预培养5 d,室温下60%PVS2预处理20 min,然后用100%PVS2于0 ℃处理40 min,投入液氮(LN)保存,24 h后在40 ℃水浴中迅速化冻,再用1.2 mol/L蔗糖培养液洗涤3次.用氯化三苯四氮唑(TTC)法检测,其存活率可达85. 62%.冻后的愈伤组织转移到MS + 6-BA 0.5 mg/L继代培养基上,在黑暗中培养生长,存活率可达76.
CHENYong(陈勇)   +2 more
doaj   +1 more source

Acoustically Assisted Heterojunction Hydrogel Promotes Angiogenesis and Tissue Regeneration by Modulating the Inflammatory Microenvironment

open access: yesRare Metals, Volume 45, Issue 8, August 2026.
ABSTRACT The healing of diabetic chronic wounds is hindered by multiple pathological barriers, including oxidative stress imbalance induced by hyperglycemia, persistent bacterial infection, and impaired neovascular function. This study developed an F127–HA–BiOCl@CeO2 (FHBC) hydrogel dressing with acoustic responsiveness, achieving tissue repair through
Ran Zhao   +8 more
wiley   +1 more source

An Ultrasound‐Triggered Antibacterial Hydrogel With Integrated Antioxidant and Angiogenic Functions for Infected Wound Care

open access: yesRare Metals, Volume 45, Issue 5, May 2026.
ABSTRACT Managing diabetic wounds requires simultaneously addressing their infectious, oxidative, and regenerative impairments. To address this, this study integrates a piezoelectric BiOCl@CuSe heterojunction (PFM‐verified) into an injectable F127/HA hydrogel, creating an ultrasound‐responsive smart dressing.
Jiayan Guo   +5 more
wiley   +1 more source

黄蜀葵茎段植株再生体系的建立和花各部愈伤组织总黄酮含量的测定比较

open access: yes四川大学学报. 自然科学版, 2012
以黄蜀葵种子获得无菌苗的茎段为外植体,研究了不同浓度激素配比对愈伤组织诱导及分化再生植株的影响.结果表明:以75%酒精30s+0.1%升汞10min灭菌黄蜀葵种子,可获得较高发芽率的无菌苗;愈伤组织最适诱导培养基为改良MS+KT0.8 mg/L+IAA0.6mg/L,经愈伤组织分化不定芽的最佳培养基为MS+6-BA3.0 mg/L+NAA0.05 mg/L;最佳生根培养基为:1/2 MS+NAA0.1 mg/L;且移栽后成活率可达95%.用紫外分光光度法测定花和各部愈伤组织总黄酮的含量,对比发现 ...
莫明雅, 王晓萌, 白洁, 陈放
doaj  

影响库拉索芦荟(Aloe vera L.)愈伤组织诱导的因素

open access: yes四川大学学报. 自然科学版, 2003
比较了不同培养基对库拉索芦荟愈伤组织的诱导效果,其中B5+6 BA2.0mg/L+2,4 D2.0mg/L+Vc5.0mg/L培养基出愈率较高,愈伤组织褐化较轻.探讨了抑制库拉索芦荟愈伤组织褐化的方法.
李琳, 叶春
doaj  

Optimization of the tannins extraction process from callus in Dimocarpus longan Lour by ultrasound-assisted extraction using response surface methodology(响应面试验优化超声波辅助提取龙眼愈伤组织中单宁的工艺)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2019
为提高龙眼愈伤组织中单宁的提取率,采用超声波辅助浸提法,基于乙醇浓度(A)、超声波功率(B)与提取时间(C) 3 个单因素试验,通过响应面试验优化设计,建立了龙眼愈伤组织单宁提取工艺。研究结果表明,龙眼愈伤组织中单宁的最佳提取条件为乙醇浓度61%、超声波功率140 W,提取时间54 min,提取温度 60 ℃,在此工艺条件下,提取量为7.53 mg · g-1。
LIUShengcai(刘生财)   +4 more
doaj   +1 more source

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