慢性乙型病毒性肝炎是目前我国最常见的传染性疾病之一,乙型肝炎病毒(HBV)感染后引起肝细胞受损,致使过多的细胞外基质(ECM)沉积,表现为肝脏弥漫性纤维结缔组织沉积即肝纤维化[1 ...
符辉, 莫青, 许铭州
doaj
【目的】 建立H5N1禽流感病毒感染BALB/c小鼠的疾病动物模型.【方法】 将100 μL H5N1禽流感病毒 原液(ELD50为10-9/0.2 mL)鼻腔接种BALB/c小鼠,设正常尿囊液组对照,接毒后14 d内每隔24 h观察一次,主要观 测指标有:临床症状?体质量和体温变化?死亡率、病理变化、病毒分离。【结果】 被感染的BALB/c小鼠的病程可以 划分为前驱期(1 ~ 2 d)、发作期(第3 ~ 7 天)、恢复期(第8 ~ 14天),发作期接毒小鼠出现各种严重的临床症状,表现出极度消瘦 ...
doaj
[Advances in clinical features, diagnosis, and treatment of pre-acute-on-chronic liver failure]. [PDF]
Nie YH, Cai DC.
europepmc +1 more source
[Comparison of antiviral efficacy of nucleos(t)ide analogue monotherapy and its combination with interferon-α in children 1-6 years-old with HBeAg-positive chronic hepatitis B]. [PDF]
Wang ZW +11 more
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[Progress in clinical research related to viral hepatitis in 2025]. [PDF]
Ding Y, Li Y, Dou XG.
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[Construction of a highly efficient HBV infection cell model based on HBV co-receptor neuropilin-1]. [PDF]
Liu ZH, He XM, Xu KX, Yu HB, Chen J.
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[Reflections on the novel definition and whether or not antiviral therapy for inactive HBsAg carrier state in the "Guidelines for the Prevention and Treatment of Chronic Hepatitis B (version 2022)"]. [PDF]
Ren S, Zheng SJ, Chen XY.
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慢病毒携带shRNA抑制CR-1基因表达对 CNE-2细胞特性的影响
【目的】 应用慢病毒介导的RNA干扰(RNAi)技术,有效沉默鼻咽癌细胞CNE-2的CR-1基因表达,研究CR-1对鼻咽癌细胞生物学行为的影响65377; 【方法】 构建针对CR-1的siRNA慢病毒表达载体[含H1启动子和绿色荧光蛋白(GFP)]65377;利用包装细胞293FT生产慢病毒,感染鼻咽癌细胞CNE-2, 流式筛选GFP阳性细胞65377;应用荧光定量PCR65380;Western blot检测病毒感染后CNE-2细胞中CR-1基因的mRNA和蛋白表达,获得干扰CR ...
doaj
[Clinical characteristics and prognosis of primary immune thrombocytopenia complicated with diffuse alveolar hemorrhage]. [PDF]
An ZY +8 more
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[The partial cure represents an intermediate endpoint on the path to a functional cure, not an ideal endpoint]. [PDF]
Zhuang H.
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