Results 11 to 20 of about 9,212 (154)
The preparation of PPy/PDA nanocomposites material and its interaction with osteoblasts [PDF]
, 2015 近年来，导电高分子纳米材料已经成为材料制备领域的研究热点之一。聚吡咯（PPy）作为导电高分子家族的重要成员，是目前研究最多并且最具有应用价值的导电高分子之一。相比于其它导电高分子，聚吡咯纳米材料不仅具有优异的稳定性、导电性以及抗腐蚀性，还具有良好的生物相容性，因此广泛应用于金属种植体涂层、药物释放载体、组织工程支架材料等生物医学领域的研究。随着人们对生物医学材料性能需求的不断提高，深入探索功能性更强、应用性更广的聚吡咯纳米复合材料已经成为聚吡咯研究的新趋势 ...
戴玲凤
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Review of additively manufactured zinc alloys by laser powder bed fusion for biomedical applications
Rare Metals, Volume 44, Issue 11, Page 8262-8291, November 2025.Graphical abstract AbstractZinc (Zn) and its alloys have emerged as promising candidates for biomedical materials, owing to their controlled degradation kinetics, intrinsic biocompatibility, and the release Zn2+ ions which are known to promote bone regeneration and tissue healing.
Xuan Yang +5 more
wiley +1 more source
Research on Roles of Nucleophosmin in Human Osteosarcoma Cells [PDF]
, 2016 Nucleophosmin作为重要的核仁磷酸化蛋白，在细胞的生命活动过程中发挥多种功能，并在多种肿瘤细胞中发生表达与细胞定位的变化。为探究Nucleophosmin在人成骨肉瘤细胞MG63中的功能作用与分子机制，本论文在成功制备Nucleophosmin兔多克隆抗体的基础上，通过构建正常定位以及胞质定位的Nucleophosmin表达载体，结合免疫共沉淀（Co-IP）、GST-pulldown、免疫荧光共定位、免疫亲和纯化和液相色谱-串联质谱联用技术（LC-MS/MS ...
石静娴
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Updated insights into the molecular pathogenesis of canine atopic dermatitis
Veterinary Dermatology, Volume 36, Issue 4, Page 375-384, August 2025.Abstract Atopic dermatitis (AD) is a common and chronic inflammatory skin disease with frequent relapses. The genomics revolution has greatly contributed and revolutionised our knowledge of human AD; understanding the molecular skin fingerprint of AD and associated pathogenic immune pathways has led to preclinical assessments of several novel ...
Frane Banovic
wiley +1 more source
Investigation of the Role and Mechanism of Rapamycin on Senile Osteoporosis via Osteocyte Autophagy [PDF]
, 2016 背景：老年性骨质疏松症患病人数逐年攀升，已成为严重的公共卫生问题。最新研究表明：骨细胞通过调节成骨细胞和破骨细胞的功能，从而主导骨重建。随着年龄增长，骨细胞凋亡增加，数目减少，导致骨代谢紊乱。细胞自噬是一种重要的生理机制，可以协助维持骨细胞活性。研究表明，因年龄增加而导致的骨细胞自噬功能障碍与老年性骨质疏松症的发病密切相关。因此，本课题以骨细胞自噬为切入点，选取经典的自噬诱导剂雷帕霉素，探究其对于老年性骨质疏松症的作用及可能机制。 方法：将52只24月龄SD大鼠随机分为雷帕霉素组和对照组 ...
罗德庆
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Ultrasound in Obstetrics &Gynecology, Volume 65, Issue 2, Page 226-234, February 2025.ABSTRACT Objectives Ultrasound is the first‐line imaging modality of the pelvis in the pediatric and adolescent gynecology (PAG) population. Ultrasound findings in pre‐ and postpubertal PAG patients differ from those in adults. Diagnostic models for adnexal pathology have not been validated in this cohort.
N. Cooper +15 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2006 目的探讨重组人胰岛素生长因子（rhIGF-1）基因对体外培养大鼠成骨细胞增殖分化的影响。方法采用酶消化法获取大鼠乳鼠颅骨成骨细胞,传至第3代进行成骨细胞鉴定,钙化结节用茜素红（ARS）染色,钙钴法显示成骨细胞中碱性磷酸酶（ALP）的表达。用脂质体法将pcDNA3.1-rhIGF-1质粒转染成骨细胞。倒置显微镜下观察细胞的生长形态,MTT法检测成骨细胞增殖情况,碱性磷酸酶及骨钙素（OCN）测定成骨细胞活性。结果建立了稳定的大鼠成骨细胞模型;pcDNA3.1-rhIGF ...
李辉南, 高凤彤, 李剑威, 王华
doaj
Effects of Jiangu Granules on the proliferation of osteoblasts through G_1/S phase cell-cycle regulated proteins [PDF]
, 2016 目的观察健骨颗粒对成骨细胞G_1/S期调控的影响,探讨健骨颗粒促进成骨细胞增殖的作用机制。方法制备健骨颗粒血清组、模型血清组和雌二醇血清组。采用酶消化法培养SD大鼠成骨细胞,健骨颗粒含药血清干预,以模型血清和雌二醇血清为对照。运用流式细胞术检测成骨细胞增殖周期,荧光定量PCR法检测成骨细胞G_1/S期调控蛋白Cyclin E、CDK2、p21和转录因子E2F-1 m RNA的表达。结果 15%的雌二醇血清与健骨颗粒血清干预48 h,成骨细胞增殖速度均明显快于模型血清组 ...
吴银生 +6 more
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Zhongguo shiyan zhenduanxue, 2012 骨肽具有调节骨代谢、刺激成骨细胞增殖、促进新骨形成等药理作用,多用于骨折和骨折手术愈合及风湿、类风湿关节炎的治疗[1]。碱性磷酸酶（ALP）和骨钙素（OC）是成骨细胞分泌的两种非胶原成分。活性成骨细胞在早期发育阶段细胞内就富含ALP,后期成熟阶段成骨细胞可产生和分泌骨钙素[2 ...
王维刚, 王志刚, 李大鹏
doaj
Identification of Candidate Plasma Altered miRNA Associated with Bone Metastasis of Lung Cancer [PDF]
, 2016 目的：应用高通量miRNA芯片及荧光定量PCR技术筛选及鉴定肺癌骨转移血浆中差异表达的miRNA。 方法：搜集2013至2015年在厦门大学附属第一医院入院的45例肺癌病人外周血标本。选取4例肺癌骨转移与4例肺癌无骨转移病人血浆，应用miRNA芯片技术对差异表达的miRNA进行筛选。应用IPA分析差异miRNA参入的信号通路。应用Real-timePCR检测候选差异表达miRNA，进行验证。 结果：通过比对，成功建立了肺癌骨转移与肺癌无骨转移血浆miRNA芯片表达谱，筛选出了12个差异表达miRNA ...
王文一
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