Results 11 to 20 of about 10,951 (190)
脉冲电刺激对改性纳米羟基磷灰石（op-HA）/PLGA复合材料表面成骨细胞粘附、增殖的影响
Zhongguo shiyan zhenduanxue, 2009 目的研究脉冲电场刺激对改性纳米羟基磷灰石（op-HA）/PLGA复合材料上培养的成骨细胞的粘附和增殖的影响。方法将组织块法原代培养的兔成骨细胞与材料体外培养,在2 V,1Hz的脉冲电刺激下,用FITC荧光染色和NIH Image J软件对图像进行数据分析观察1 d,3 d和7 d成骨细胞在材料表面生长的形态、单个细胞面积比;MTT法检测各组吸光度（A）值。结果随着培养时间的增长,细胞形态由三角形、梭形转变为多角形,单个细胞面积比逐渐增大。op-HA/PLGA表面的单个细胞面积比高于PLGA的 ...
于婷 +6 more
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Zhongguo shiyan zhenduanxue, 2005 目的研究成骨细胞特异性因子（Osf2）调控骨髓间充质干细胞（MSCs）定向分化为成骨细胞（OB）的分子机制。方法用Percoll密度梯度离心法分离获得大鼠的MSCs,通过细胞免疫化学、免疫荧光、透射电镜及流式细胞仪等方法进行鉴定。构建真核表达载体pcDNA3.1（+）/Osf2,通过脂质体系统转染传代培养后的MSCs,用Northern Blot检测三种OB细胞外基质蛋白mRNA的表达情况。结果转染后的MSCs都表达OB特异性基质蛋白骨钙素、骨桥接蛋白和I型胶原 ...
王溪原 +6 more
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[miR-488-5p promotes osteogenic and neurogenic differentiation of rat bone marrow mesenchymal stem cells and enhances neuralized bone regeneration]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZeng L +5 more
europepmc +3 more sources
Zhongguo shiyan zhenduanxue, 2007 目的探讨兔骨髓基质细胞（BMSCs）的体外分离培养及定向诱导成骨的方法。方法选择10周龄新西兰兔,抽取其股骨大转子部骨髓,体外分离纯化得到BMSCs,再利用条件培养液将其向成骨方向定向诱导培养。采用碱性磷酸酶（ALP）和冯库萨（Von Kossa）染色方法,鉴定其成骨性能。结果BMSCs在培养皿中贴壁生长、增殖,经ALP和Von Kossa染色证实其有成骨潜能。结论兔BMSCs的体外培养增殖能力强,能诱导分化为成骨细胞,可以作为骨组织工程的种子细胞。
李树春, 陈钢, 李文革, 江智茂
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Successful surgical removal of a pheochromocytoma in a mare via trans‐costal approach
Equine Veterinary Journal, Volume 55, Issue 6, Page 1012-1020, November 2023., 2023 Abstract Background Pheochromocytomas have been previously reported in horses, but successful antemortem diagnosis and surgical removal without recurrence of clinical signs have not been described. Objective To report the clinical presentation, diagnostic evaluation, surgical technique, anaesthetic management and post‐operative care of a mare diagnosed
Thomas J. O'Brien +6 more
wiley +1 more source
Advances in Chinese medicine-mediated AMPK/mTOR signaling pathway for the treatment of osteoporosis [PDF]
, 2023 Osteoporosis （OP） is a systemic bone disease and a common orthopedic disorder in elderly patients. High morbidity， disability and mortality rates and medical cost of OP bring huge burden to the patients and their family.
Zhang Wenhao, Yi Lin, Xiang Wenyuan, Remira·Aimaiti, Deng Yingjie, Liao Jun, Liang Zhiquan, Li Leijiang, Fang Rui
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Study of the effect of Strong-bone granules on the differentiation of ROS1728 osteoblasts with silenced ER expression [PDF]
, 2017 目的探讨健骨颗粒对成骨细胞中ERalpha介导的TERT信号通路的调控作用。方法采用雌激素受体拮抗剂ICI182780(Faslodex)阻断成; 骨细胞中雌激素受体alpha(ERalpha)的表达,建立ER抑制的大鼠成骨细胞株ROS1728细胞模型,采用酶联免疫吸附法(ELISA)检测成; 骨细胞液中碱性磷酸酶(alkaline phosphatase,ALP) 、骨钙素(osteocalcin,BGP); 、Ⅰ型胶原(collagen I,Col Ⅰ)的含量。采用实时荧光定量SYBR ...
吴银生 +6 more
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Research progress in the roles of Notch signaling pathway during fracture healing [PDF]
, 2023 Fracture is the most common large-organ injury in humans. It takes several months or even longer from the onset to the completion of bone reconstruction. In severe cases, delayed healing or even bone discontinuity can occur.
GUO Liqiang, SHU Bing, ZHAO Shitian
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Effects of olive oil on osteoporosis after artificial menopause [PDF]
, 2015 随着妇科恶性肿瘤发病率的逐年上升及越趋年轻化，给社会和家庭带来了巨大的经济和精神负担。妇科恶性肿瘤主要以手术为主，并辅以放化疗。随着手术切除双侧卵巢及放化疗对卵巢损害的发生，将会导致雌激素急剧下降，从而增加骨质疏松症的发病率。目前激素替代疗法（hormonereplacementtherapy，HRT）广泛用于防治人工绝经后骨质疏松症，但是有临床大样本研究表明，HRT虽然能够减缓绝经后骨质疏松症的发生，但也增加了心血管疾病、乳腺癌、中风、糖尿病、宫颈癌、子宫内膜癌、卵巢癌等的发病率 ...
刘慧兰
core
Pharmacological Research Progress of Cistanches Herba [PDF]
, 2017 目的探讨肉苁蓉药理作用。方法收集近几年国内外对肉苁蓉药理作用的研究资料,并对其进行综述。结果现代药理研究表明,肉苁蓉有润肠通便、保肝、抗骨质疏松、抗氧化、抗衰老、抗疲劳等作用。结论肉苁蓉药理作用广泛,为其临床应用提供一定的参考依据。Objective Discuss deeply the pharmacological action of cistanches herba.
方鉴
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