Applications and Challenges of Artificial Intelligence in Early Diagnosis and Precise Treatment of Lung Cancer [PDF]
The incidence and mortality of lung cancer are extremely high, and the 5-year survival rate of patients after diagnosis is less than 20%; as such, early screening and intervention are important.
Bojiang CHEN, Shouyu ZHANG, Weimin LI
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SYBR GreenⅠ实时RT-PCR定量检测前列腺癌患者外周血DD3mRNA
Zhongguo shiyan zhenduanxue, 2007 目的建立一种新的方法定量检测前列腺癌（PC）患者外周血DD3 mRNA方法扩增LNCaP细胞株阳性表达的DD3mRNA,并以其扩增产物为外标准,通过优选引物及优化PCR的各项参数,建立了SYBR Green实时RT-PCR定量检测PC患者外周血DD3 mRNA的方法,并以此方法检测了健康男性志愿者、良性前列腺炎（BPH）患者和PC患者外周血DD3 mRNA。结果所建立的SYBR Green实时RT-PCR方法最少可检测到10拷贝的核酸,在每反应108-103copies/ml范围内 ...
张黎明 +4 more
doaj
Research advance in genetic diagnosis of monogenic inherited diseases [PDF]
, 2016 单基因遗传病(简称单基因病)种类、分型繁多,常规诊断难以确诊,而基因诊断技术在遗传病特别是单基因病的确诊、分型等方面都发挥着不可或缺的作用。近一、二十年来,基因诊断技术进展迅猛,各种检测方法层出不穷。本文重点围绕基因诊断技术的最新进展进行综述,以期对临床诊断和预防工作提供一些有益的启示。Monogenic diseases are caused by inheritance of single mutated gene, but the type of the diseases are ...
梁宇静, 郭东炜, 郭奕斌
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Rapid prenatal diagnosis of chromosomal aneuploidy using real-time multiplex ligation-dependent probe amplification （real-time MLPA） [PDF]
, 2008 染色体非整倍体是一类发病率高，病征严重的遗传性疾病。对于大多数此类病征目前尚无有效治疗的手段。因此，快速、准确、高通量地对染色体非整倍体进行产前诊断对于减少患病胎儿的出生、提高人口素质具有十分重要的意义。本论文运用实时荧光多重探针连接再扩增技术以及相关的数据分析方式，致力于实现对染色体非整倍胎儿的快速产前诊断。 第一章，综述了染色体非整倍体的产前诊断现状，介绍了传统细胞生物学诊断方法以及新兴分子生物学诊断方法的技术特点与应用现状。针对目前分子生物学产前诊断技术的优缺点 ...
郭奇伟
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The Latest Research Progress on Cell-Free DNA and Prospects of Its Forensic Application [PDF]
In recent years， with the continuous progress of DNA extraction and detection technology， cell-free DNA（cfDNA）has been widely used in the life science field， and its potential application value in forensic identification is becoming more and more obvious.
Wen-jing HU, Ting-ting YANG, Ya-ya WANG, Jiang-wei YAN
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Integrative Analysis of Prostate Cancer Methylome and Smoking-induced Transgenerational Epigenomic Reprogramming [PDF]
, 2013 Epigenetic factors such as DNA methylation, histone modification and noncoding RNAs are highly associated with early developmental processes, later environmental adaption and diseases development such as cancer.
Gu, Lei
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Zhongguo shiyan zhenduanxue目的 基于二代测序平台，本研究开发了一种新型染色体异常检测技术—CNV-plus,旨在探究该技术的检测性能及优势，为临床中拷贝数的高精度和全面检测提供新方法。方法 使用CNV-plus对共173例流产组织样本进行染色体拷贝数检测，并通过CNV-seq和STR两种方法的验证和补充，对比分析检测结果的可检出变异类型、检测一致性及检测精度。结果 CNV-plus技术与CNV-seq对CNV有100%的检测一致性，且检测精度更高，排除母源污染后，CNV-plus与STR对三倍体有100%的检测一致性。与CNV ...
庄燕燕 +8 more
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Exome sequencing detect susceptibility gene mutations IFNA4, IFNA10 in Chinese Crohn’s disease patients [PDF]
, 2013 背景克罗恩病的发生与遗传因素密切相关，近年来在中国的发病率呈逐年上升趋势，但对其发病机制的理解还十分有限。克罗恩病的基因异质性和种族差异十分突出，但中国人群特异性的易感基因尚未明确。 目的本课题首次对中国克罗恩病病例进行外显子高通量测序，在全外显子范围内寻找克罗恩病新的突变基因位点，探讨易感基因位点与克罗恩病内科药物疗效的关系，并利用体内、体外实验对易感基因进行功能验证，探索其致病机制。 方法我们对4例中国克罗恩病患者外周血样本进行全基因组外显子高通量测序，筛选出共有突变位点 ...
王欢欢
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Comparison of DNA and RNA extraction efficiency from blood [PDF]
Objective·To comprehensively evaluate the efficiency of different kits and methods for DNA and RNA extraction from blood samples.Methods·A total of 145 blood samples were collected, including those from patients with Alzheimer's disease (20 cases ...
HAN Da +5 more
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浙江大学学报. 农业与生命科学版Examining the genetic diversity of Dendrobium officinale from different geographical origins is crucial for advancing the understanding of the origin and evolutionary divergence of this species, and provides a valuable theoretical basis for the ...
周宇(ZHOU Yu) +4 more
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