Results 61 to 70 of about 29,324 (153)

Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity

open access: yesNew Phytologist, Volume 251, Issue 3, Page 1283-1293, August 2026.
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng   +4 more
wiley   +1 more source

植物油提取新工艺得到的五味子提取物对小鼠睡眠影响的实验研究

open access: yesZhongguo shiyan zhenduanxue, 2011
目的观察植物油提取新工艺得到的五味子提取物的改善睡眠作用。方法采用植物油提取新工艺得到五味子提取物,昆明种雄性小鼠随机分为对照组及五味子提取物低、中、高剂量组,对照组给予橄榄油20 ml/kg/d灌胃,五味子提取物低、中、高剂量组分别给予五味子提取物150、3006、00 mg/kg/d,连续灌胃15 d,通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏期实验,观察对小鼠直接睡眠、阈剂量戊巴比妥钠睡眠时间 ...
徐仲航, 金向群
doaj  

响应面优化氯化胆碱-丙三醇体系提取芝麻蛋白工艺条件 "Response surface optimization of the extraction process conditions for sesame protein using choline chloride-glycerol system"

open access: yesZhongguo youzhi
为提高芝麻油副产物芝麻饼粕中蛋白质的综合利用率,以芝麻粕为原料,选用氯化胆碱-丙三醇(物质的量比1∶ 3)体系(低共熔溶剂,DES)提取芝麻蛋白,通过单因素试验和响应面试验优化提取工艺条件,分析最优条件下提取的芝麻蛋白的功能特性,并采用傅里叶变换红外光谱和扫描电子显微镜对芝麻蛋白进行结构表征,同时与传统碱溶酸沉法提取工艺进行对比。结果表明:DES提取芝麻蛋白的最优条件为DES含水量10%、固液比1∶ 29.4、提取温度59.0 ℃、提取时间1 h、搅拌速度 750 r/min,在此条件下芝麻蛋白提取率为(
曹培换1,张晨霞1,刘燕2,马宇翔1,刘华敏1,郑永战1,3 "CAO Peihuan1,ZHANG Chenxia1,LIU Yan2,MA Yuxiang1, LIU Huamin1,ZHENG Yongzhan1,3"
doaj   +1 more source

Moving in Cycles: Explaining the Failure of Militarization to Address Illegal Mining in Ghana

open access: yesPolitics &Policy, Volume 54, Issue 4, August 2026.
ABSTRACT Mining contributes to Ghana's economic growth but also imposes significant social costs—land dispossession and unemployment—which have fueled the rise of illegal mining (galamsey). Galamsey, which intensified during the commodity boom of the 2010s, provides livelihoods while simultaneously worsening environmental degradation and social ...
Sulemana Alhassan Saaka, Phil Faanu
wiley   +1 more source

Conflicts Over Wage Floors: What Led to Minimum Wage Increases in the US States Between 2013 and 2022?

open access: yesPolitics &Policy, Volume 54, Issue 4, August 2026.
ABSTRACT After 2013, the number of statewide minimum wage increases in the United States surged. However, not every state followed the trend. Some refrained from minimum wage legislation completely. This article examines the causes of differences in state minimum wage policies from 2013 to 2022, taking a qualitative perspective on minimum wage politics
Emanuel Schuller
wiley   +1 more source

Channeling Action: How Civic Recreation Organizations Shape Civic Capacity and Engagement

open access: yesPolitics &Policy, Volume 54, Issue 4, August 2026.
ABSTRACT In this paper, we examine the individual‐level and organizational‐level implications of participation in a particular type of civic association—a civic recreation organization. We deploy a small‐n comparison group design, comparing data within and across members of a civic recreation organization with varying degrees of involvement to ...
Emma Scheetz, David Carter
wiley   +1 more source

紫茎泽兰提取物抑制食源性致病菌的研究

open access: yes四川大学学报. 自然科学版, 2009
用黄芪,苦参,狼毒提取物为阳性对照对紫茎泽兰提取物进行抑菌活性比较。并利用液液萃取、色谱法对紫荆泽兰醇提物进行分离精制。结果表明,紫茎泽兰醇提物具有明显抑菌效果,且效果优于狼毒提取物和黄芪提取物;对紫茎泽兰醇提物进行分离精制及抑菌试验,结果显示其乙酸乙酯萃取物柱层析组分SF4-2抑菌活性最强,能显著抑制沙门氏菌,蜡样芽孢杆菌,金黄色葡萄球菌和枯草芽孢杆菌生长,可用于食源性抗菌制剂的开发利用。
王婧   +6 more
doaj  

Epitaxial Growth Mechanism and Magnetic Properties of Heterogeneous Core–Shell Grains in (Nd, Y)‐Fe‐B Alloys

open access: yesRare Metals, Volume 45, Issue 7, July 2026.
ABSTRACT The construction of core–shell grains can enhance the coercivity of Nd2Fe14B magnets. However, it is a significant challenge to elucidate the formation of these core–shell grains particularly regarding the spontaneous core–shell structure in Y‐substituted Nd2Fe14B alloys.
Sining Fan   +13 more
wiley   +1 more source

植物甾醇提取纯化方法研究进展Research progress on extraction and purification methods of phytosterols

open access: yesZhongguo youzhi
为了提高植物甾醇的提取效率和纯度,对植物甾醇的结构和来源,提取纯化方法及其辅助技术的原理、种类、特点以及应用等进行归纳分析,并对今后的研究方向提出了建议。植物甾醇来源广泛,但传统的植物甾醇提取纯化方法如溶剂萃取法等存在收率低、纯度低以及影响环境等缺点,具有一定局限性。新型的植物甾醇提取纯化方法如超临界CO2法等具有操作简单、无毒、产品纯度高等优点,是一种环保高效的提取方法。辅助提取技术如酶、微波、超声波和磁场等能够优化对植物甾醇的提取纯化工艺,提高提取纯化效率。通过将传统方法进行改良、与新型方法结合 ...
王媛媛,马改琴,李跃凡,李琪,高媛,于修烛WANG Yuanyuan, MA Gaiqin, LI Yuefan, LI Qi, GAO Yuan, YU Xiuzhu
doaj   +1 more source

基于复合特征的GIS振动信号特征提取方法

open access: yesGaoya dianqi, 2021
对GIS的振动信号进行分析可以及时有效的发现设备中存在的故障。在GIS设备的故障诊断中,特征提取是最为关键的步骤,文中针对单一种类的特征提取方法提取到的特征容易缺乏特异性这一缺点,提出了基于复合特征的GIS振动信号特征提取方法。首先将奇异值分解和基于总体最小二乘准则的旋转不变技术这两种时频法结合起来对振动信号中具有明确物理意义的特征量进行提取,对于没有明确物理意义的特征量,通过数据序列法中的变分模态分解和多重分形来对其进行提取;然后将这两种特征量组合得到新的特征量 ...
马星光   +5 more
doaj  

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