Results 41 to 50 of about 758,549 (212)
Zhongguo shiyan zhenduanxue, 2006 目的为寻找一种高效、简便、实用的石蜡组织中DNA提取方法,本研究比较了三种不同的DNA提取方法对DNA质量的影响。方法选取淋巴相关组织的蜡块21例。三种DNA提取方法分别是简单消化煮沸法,酚/氯仿提纯法和试剂盒法。通过提取DNA的浓度和纯度、PCR扩增保守序列β-globin等方法,比较三种DNA提取方法的优劣。结果试剂盒法提取样品OD260/OD280平均比值位于1.61.8之间;提纯法位于1.31.5之间;简单消化煮沸法位于1.21.3之间。三种方法中,试剂盒法提取的DNA最为稳定 ...
齐宗利 +4 more
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Zhongguo shiyan zhenduanxue, 2020 目的探讨在高原地区采用AMBION抽提试剂盒提取RNA的最佳条件。方法采用AMBION抽提试剂盒,对126份大鼠耳蜗(每份耳蜗分为2组,实验组和对照组)提取RNA,实验组采用AMBION抽提试剂盒优化条件提取RNA,对照组按照AMBION抽提试剂盒说明书提取RNA。结果实验组和对照组均能成功提取大鼠耳蜗组织RNA,总RNA的提取量分别为:0.896±0.348μg,0.536±0.404μg(P>0.05);实验组RNA浓度高于对照组,分别为:0.147±0.047μg/μl,0.072±0.022μg/
师晶晶 +5 more
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Institutional lessons from the Nordic management model
Conservation Biology, EarlyView.Abstract To improve wildlife management outcomes, in terms of reaching management goals and increasing legitimacy, Nordic countries have developed institutional systems involving stakeholders that aim for a more inclusive and participatory model.
Jens Nilsson +2 more
wiley +1 more source
"fordi før så skreiv æ Å-G". En studie om andreklassingers valg av stavemåter.
, 2023 The purpose of this master's thesis has been to look at the choices students in Year 2 make when spelling words. The students participating in this research are in the transition between phonological and orthographic writing, and the study will focus on how these students adopt different spelling strategies. The assignment's data material is taken from
openaire +1 more source
四川大学学报. 自然科学版, 2013 用水蒸气蒸馏法、超声辅助浸提法和索氏提取法提取山桐子果实中的挥发油,用GC-MS分离鉴定其成分并测定各成分的相对含量.水蒸气蒸馏法、超声辅助浸提法和索氏提取法分别分离鉴定出64、50和57个组分,共有化合物38种,其中主要成分为亚油酸、棕榈酸、油酸、角鲨烯、椰子醛和γ-维生素E等.3种提取方法所得山桐子果实挥发油成分有共同特征,也存在着差异,相较之下,水蒸气蒸馏法提取的挥发油成分最多,索氏提取法提取挥发油率最高.
叶扬 +5 more
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Conservation Biology, EarlyView.Abstract Reversing ongoing biodiversity loss requires effectively managing anthropogenic threats. Although conservation actions can mitigate these threats, prioritizing and targeting such actions remain challenging because species respond differently depending on the nature of the threats and their biological traits.
Camila Guerrero‐Pineda +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2011 目的观察植物油提取新工艺得到的五味子提取物的改善睡眠作用。方法采用植物油提取新工艺得到五味子提取物,昆明种雄性小鼠随机分为对照组及五味子提取物低、中、高剂量组,对照组给予橄榄油20 ml/kg/d灌胃,五味子提取物低、中、高剂量组分别给予五味子提取物150、3006、00 mg/kg/d,连续灌胃15 d,通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏期实验,观察对小鼠直接睡眠、阈剂量戊巴比妥钠睡眠时间 ...
徐仲航, 金向群
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Conservation Biology, EarlyView.Abstract The sustainability of area‐based conservation initiatives depends on their value to society. Conservation often focuses inside protected areas, but the impacts of protected areas beyond their boundaries (spillovers) are critical for their social license.
Ilaria Gliottone +5 more
wiley +1 more source
, 2014 本发明公开了一种易变山羊草的咖啡因提取物及其提取方法与应用，属于生物工程领域。本发明中易变山羊草的咖啡因提取物的提取方法的具体步骤如下：取易变山羊草根部清洗后切成片，在80℃烘箱中烘干6小时彻底去除水分，然后用粉碎机粉碎，过60目筛后，把10克粉碎的易变山羊草根部样放入50毫升的萃取釜中，采用无水乙醇作为夹带剂，在CO2提取仪中30MPa，60℃萃取2h，其中CO2流量控制在1.5LPM，获得易变山羊草的咖啡因提取物。本发明研究表明＞0.1％浓度的咖啡因对植物病原线虫有生长有抑制作用 ...
郑明辉 +6 more
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Pest‐preservation paradox among insects
Conservation Biology, EarlyView.The pest‐preservation paradox is a non‐binary perspective that insects range from those that are harmful and requiring management on the one side, to those requiring conservation intervention on the opposite end of the spectrum. Importantly, the vast majority of insects occur somewhere in the center of the two extremes, and their position along the ...
Michael J. Samways +3 more
wiley +1 more source