, 2001 本研究采用化学分析、室内土壤培养试验、渗漏池渗漏试验和不同作物田块调查相结合的方法，系统研究了氮肥在灌淤土(渭干河流域)中的转化过程和淋洗现状及其影响因素。通过本研究，掌握了灌淤土中氮肥的转化过程和淋洗现状，并对其进行评价，主要结果如下：1.尿素态氮的比色测定方法研究改进后的测定方法对尿素态氮的检测有回收率高、重复性好、反应快速和成本低的优点，适合于大批量样品的测试。2M NaCL-PMA同时适用于硝态氮、亚硝态氮和铵态氮的浸提测定。2.氮肥在灌淤土中的转化及其影响因素温度对尿素态氮转化速度有显著的影响，
王平
core
RhoA/Rho激酶信号通路在血管紧张素Ⅱ刺激心肌成纤维细胞增殖和胶原合成中的作用
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨RhoA/Rho激酶信号通路在血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)刺激心肌成纤维细胞(cardiac fibroblasts,CFBs)增殖和胶原合成中的作用。【方法】采用胰酶消化、差速贴壁法培养新生SD大鼠CFBs,并用AngⅡ诱导CFBs增殖和胶原合成。采用四氮唑盐比色法测定细胞增殖,羟脯氨酸法测定CFBs胶原含量,RT-PCR检测RhoA/Rho激酶mRNA的表达,Western blot检测肌球蛋白结合亚基磷酸化(phosphorylation of myosin ...
汪祥海 +5 more
doaj
[Extracellular matrix materials and tissue regeneration and repair]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiZhao L, Xie H.
europepmc +1 more source
[Research progress in the regulation of allergic rhinitis pathogenesis by the NRF2 pathway]. [PDF]
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za ZhiLi Q, An Y, Li T, Zhou J, Wang W.
europepmc +1 more source
[Role of Trained Immunity in the Association Between Periodontitis and Rheumatoid Arthritis]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanYou R, Cui Y, Sun J.
europepmc +1 more source
[Molecular imprinting strategies and advances targeting biomembranes]. [PDF]
Se PuYuan XT, Wang L, Chen LX, Hu LH.
europepmc +1 more source
[The role of polyunsaturated fatty acid lipid peroxidation in ferroptosis after intracerebral hemorrhage: a review of mecha-nisms and therapeutic implications]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanGuo M, Zhao G, Cai Z, Zhang Z, Zhou J.
europepmc +1 more source
[Traditional Chinese medicine for regulating glycolysis to remodel the tumor immune microenvironment: research progress and future prospects]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoHe S +8 more
europepmc +1 more source
[Mechanisms by which the gut microbiota regulates depressive disorder via the tryptophan metabolic pathway]. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue BanDU J +6 more
europepmc +1 more source
野生动物学报为研究梅花鹿（Cervus nippon）DLX5基因的结构和功能，进一步探究其与梅花鹿茸角骨化机制间的关系，采用RT-PCR技术对梅花鹿DLX5基因进行克隆，获得包含全部编码区的cDNA序列，对该基因的氨基酸序列进行生物信息学分析并构建系统进化树，通过KEGG富集分析其信号通路，运用实时荧光定量RT-PCR检测该基因在鹿茸生长不同时期的表达情况。结果表明：梅花鹿DLX5基因编码区长为870 bp，共编码289个氨基酸。DLX5蛋白为可溶性的不稳定蛋白，有2个保守结构域，主要定位于细胞核 ...
王鹏 +5 more
doaj