矢车菊素－３－葡萄糖苷通过降低ＳＴＡＴ３活化抑制ＴＮＦ－α诱导的小鼠血管平滑肌细胞增殖
Zhongshan Daxue xuebao. Yixue kexue ban, 2012 【目的】 探讨矢车菊素－３－葡萄糖苷（Ｃ３Ｇ）对ＴＮＦ－α诱导的血管平滑肌增殖的影响及可能的机制。【方法】 Ｃ５７ＢＬ／６Ｊ小鼠主动脉平滑肌细胞（ＶＳＭＣ） 购于ＡＴＣＣ，体外培养后以Ｃ３Ｇ对细胞进行预处理后观察ＴＮＦ－α的促细胞增殖作用；Ｄｉｈｙｄｒｏｅｔｈｉｄｉｕｍ （ＤＨＥ）荧光染色检测ＶＳＭＣ在ＴＮＦ－α作用下的活性氧（ＲＯＳ）生成情况；实时定量ＰＣＲ检测细胞内ＮＡＤＰＨ氧化酶活化蛋白１（ＮｏｘＡ１）的ｍＲＮＡ水平；蛋白质印迹法检测细胞内ＮｏｘＡ１、信号转导与转录激活因子３ （ＳＴＡＴ３ ...
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