Electrochemiluminescence Biosensors Based on Enzyme Crosslinked Ru(bpy)32+ -doped SiO2 Nanoparticles [PDF]
电致化学发光(ECL)具有连续可测、高灵敏度、稳定、方法简单等特点,因此被广泛应用于分子生物学、药学、化学和环保等领域。联吡啶钌由于具有水溶性好,化学性能稳定,氧化还原可逆,发光效率高和激发态寿命长等特点而成为ECL研究最多应用最广的ECL发光物质。将联吡啶钌包埋于二氧化硅纳米颗粒的内部能够使其发光免受外界环境的影响,且增加了光稳定性和增强了发光信号,同时,由于表面的二氧化硅层容易修饰一些官能团,如氨基、巯基、羰基等,扩大了联吡啶钌ECL在生物分析领域的应用。本论文工作将酶交联于RuDS的表面 ...
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