Results 51 to 60 of about 8,937 (167)
Biochemical characteristics and function studies of Esx-1 secretion-associated protein EspL and EspG1 [PDF]
, 2016 由巨噬细胞等人体免疫细胞聚集形成的肉芽肿是结核病的形成标志，肉芽肿的形成在结核分枝杆菌的致病过程中扮演着重要角色，它使被感染的宿主与结核分枝杆菌融合在一起。结核分枝杆菌通过特异性的蛋白分泌系统Esx-1分泌系统将具有毒性作用的毒力因子通过高疏水性的细胞膜分泌到胞外。这些毒力因子与宿主中肉芽肿的形成以及结核分枝杆菌毒性作用有关。EspL是结核分枝杆菌Esx-1分泌系统中的一个重要的蛋白，已有研究表明EspL可能通过影响另外两个毒力因子EspE和ESAT-6的分泌而调控肉芽肿的形成 ...
田双良
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Policy Studies Journal, Volume 53, Issue 4, Page 994-1013, November 2025.Abstract Defined by threat, urgency, and uncertainty, crises produce opportunities for government leaders to exploit and create meaning around their policy decisions in such unstable circumstances. In narrating their preferred policy solutions, one of the tools governments can use is relying on evidence‐based information.
Laura Mastroianni, Stefania Profeti
wiley +1 more source
Cloning and expression patterns of stem cells induction-associated pluripotency factors in large yellow croaker Larimichthys crocea [PDF]
, 2016 通过iPSCs技术获得iPSCs，建立个体特异的多能干细胞系，可为生长发育、种质资源保护、疾病防治及药物筛选研究等提供宝贵的实验材料，是一条新的从根本上解决大黄鱼养殖中的各种难题的有效策略模式。其中，多能性因子对iPSCs技术的研究是必不可少的，它们在调控胚胎干细胞（ESCs）的自我更新和多能性、原始生殖细胞（PGCs）的形成、早期胚胎发育、生殖细胞发生、器官发生、再生及增殖、生长、分化、凋亡等细胞过程等方面发挥重要作用。然而关于大黄鱼多能性因子的研究尚属空白。本文采用SMART ...
姜永华
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Exploring the importance of aromatic plants' extrafloral volatiles for pollinator attraction
New Phytologist, Volume 248, Issue 2, Page 517-528, October 2025.Summary Aromatic plants occur in many plant lineages and have widespread ethnobiological significance. Yet, the ecological significance and evolutionary origins of aromatic volatile emissions remain uncertain. Aromatic emissions have been implicated in defensive interactions but may also have other important functions.
Aphrodite Kantsa +3 more
wiley +1 more source
Research on Roles of Nucleophosmin in Human Osteosarcoma Cells [PDF]
, 2016 Nucleophosmin作为重要的核仁磷酸化蛋白，在细胞的生命活动过程中发挥多种功能，并在多种肿瘤细胞中发生表达与细胞定位的变化。为探究Nucleophosmin在人成骨肉瘤细胞MG63中的功能作用与分子机制，本论文在成功制备Nucleophosmin兔多克隆抗体的基础上，通过构建正常定位以及胞质定位的Nucleophosmin表达载体，结合免疫共沉淀（Co-IP）、GST-pulldown、免疫荧光共定位、免疫亲和纯化和液相色谱-串联质谱联用技术（LC-MS/MS ...
石静娴
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The Two Faces of Introgression in Conservation Biology 基因渗入在保育生物学中的双重面貌
Integrative Conservation, Volume 4, Issue 3, Page 339-344, September 2025.Conservation biologist need to take into account the various aspects of introgression in their attempts to protect the species integrity and evolutionary potential of threatened species. To do so, they have to overcome the bias created by the Mayrian species concept that considers species as having distinct gene pools.
Harald Schneider, Charles H. Cannon
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2013 20年来,医学信息量增长速度之快,使得最勤勉的学生,要赶上信息的"脚步"不是一件容易的事情,全面高效地学习是时代所趋,也是成为一个合格医生的核心因素。传染病学作为临床医学专业的必修课,传统教学法以病原体的生物学分类为基础单病种进行讲解,存在着显而易见的缺陷和不足。这与当前素质教育的主流思想相违背,与传染病学学科发展及培养高素质医学人才已不相适应,传染病教学模式的改革势在必行[1 ...
王静霞 +3 more
doaj
, 2015 为了建立在姜黄素诱导肿瘤细胞凋亡过程中以Nucleophosmin为中心的分子调控网络，阐明Nucleophosmin的表达和定位变化在肿瘤细胞诱导凋亡过程中的调控作用机制，本论文在成功制备Nucleophosmin兔多克隆抗体的基础上，运用Westernblot和免疫荧光标记技术（IF）分析Nucleophosmin在姜黄素诱导凋亡前后的人肝癌SMMC-7721细胞中的表达和定位变化，同时构建带有Flag标签的正常定位（pBobi-cmv-NPM）以及核仁定位信号突变（pBobi-cmv-NPM ...
李晓
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Study on Ensemble Algorithm for Multi-class Gene Microarray Datasets [PDF]
, 2015 集成学习是当前机器学习领域的一个研究热点，具体到多分类问题，旨在通过一组差异的分类器共同解决起初的多分类问题，然后经过大多数投票等策略将各个分类器的输出结果进行融合。集成多分类算法相比于单个的优秀分类器往往性能上更准确、更稳定，同时还具有更强的泛化能力。在解决多分类问题时，基于纠错输出编码算法（ECOC）。这是解决多分类问题的一种灵活、高效的算法框架，关键要点是将多分类转变为多个二分类问题。此外，遗传规划算法可用于解决二分类问题，通过进化计算得到准确的分类规则。本文在已有的研究基础上 ...
曾志浩
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Functional Ecology, Volume 39, Issue 9, Page 2327-2341, September 2025.Read the free Plain Language Summary for this article on the Journal blog. Abstract While elevational patterns of soil bacterial and fungal communities in mountain ecosystems have been well documented, the assembly processes of soil archaeal and protistan communities, and their linkage with ecosystem multifunctionality (EMF), remain poorly understood ...
Bao‐Min Yao +14 more
wiley +1 more source