Results 121 to 130 of about 2,341,099 (172)
, 2011 本发明涉及植物细胞培养技术，具体的说是一种利用三尖杉液体悬浮培养细胞生物合成松香烷二萜类化合物的方法。将三尖杉细胞置于其常用培养基中培养，细胞接种量为50～150g/L湿重，在细胞指数生长初期，于每升培养基中同时加入终浓度为10～2000μM的诱导剂和50～200g的吸附剂，所述诱导剂为非生物诱导剂或真菌诱导子。本发明为生物合成方法，方法稳定，环境友好；产物完全被吸附到吸附剂上，大大简化了分离纯化的操作流程，不仅降低了成本，而且易于实现工业化生产。同时 ...
张卫, 徐晓辉
core
, 2015 磷酸烯醇式丙酮酸(PEP)的羧化是大肠杆菌合成丁二酸的关键反应. 由于PEP羧化激酶(PCK)催化过程伴随三磷酸腺苷(ATP)的产生, 故更有利于菌体生长和丁二酸生成, 但是PCK对于底物HCO_3~-亲和性较弱, 也存在着催化效率低的问题. 针对该问题, 本研究构建了产丁二酸工程菌BS101 (W3110△ldhA△pflB::Kan/pTrcHisA-130ZPCK), 探讨酶的表达水平、HCO_3~-的供应方式及浓度对PCK催化效率的影响, 优化能量代谢途径, 提高菌株积累丁二酸的能力. 结果表明,
杨茂华, 邢建民, 刘谊兰, 白冰
core
[Correlation between trimethylamine-N-oxide and cardiovascular metabolic markers]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZhang H +18 more
europepmc +1 more source
[Microenvironmental dysregulation and advances in functional biomaterial-based interventions for diabetic bone defect repair]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiYang K, Sun Y, Bai R, Yang Y.
europepmc +1 more source
[IgG4-related disease presenting with involvement of the eyes, pituitary gland and nervous system: A case report]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanHao C, Li Z, Qin L, Yin Y.
europepmc +1 more source
[Research progress of cementless prosthesis in total knee arthroplasty]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiTai W, Qi X, Wang B, Chai W.
europepmc +1 more source
[Research progress on the application of stem cells and their derivatives in chronic wound repair]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiWu RX +8 more
europepmc +1 more source
[Pediatric thunderstorm asthma in China: epidemiological characteristics and comprehensive management]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiHan P, Shen KL.
europepmc +1 more source
[Research progress on the application of pollen in the biomedical field]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiBai L, Wang Y, Yang L, Wang Y, Sun Y.
europepmc +1 more source