摘 要: 【目的】 探讨AKT不同亚型AKT1和AKT2在成人脂肪干细胞成脂分化过程的表达及意义?【方法】取生长状态良好的P3代的成人脂肪组织来源的间充质干细胞(hadsc),以负载AKT1-shrna和AKT2-SHRNA的慢病毒载体分别转染hadsc,获得稳定干扰AKT1和AKT2的细胞株(分别设为AKT1-3组和AKT2-1组),转染非干扰序列NTC-shrna的设为对照组?将上述细胞分别进行成脂肪诱导,观察脂滴的形成,并检测相关成脂肪基因的表达和成脂肪关键转录因子pparγ的表达?
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[Rapid determination of lubabegron residues in animal-derived foods by ultra performance liquid chromatography- tandem mass spectrometry]. [PDF]
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Liao Y, Xiao TT.
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金属氧化物避雷器(Metal Oxide Arresters,MOA)由于其优异的非线性特性在电力系统中得到了十分广泛的应用,对其运行状态进行有效的在线监测是保障电力系统可靠供电的关键。针对受避雷器滞回特性和不对称相间干扰影响,电网中使用的基于容性电流补偿法的避雷器监测装置存在测量误差的问题,文中使用基于电压过零点的微分方法计算避雷器的晶界电容和相间耦合电容,实现了对三相避雷器的阻性电流进行提取,并与容性电流补偿法进行了对比。通过仿真和实测进行验证,结果表明本方法能够消除滞回特性 ...
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Huang H, Chen H, Qiao L.
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