目的筛选与弱精子症发生相关的基因。方法采集正常男性和弱精患者精液精子,非连续梯度离心法分离纯化精子,收集95%percoll以下和57%与76%Percoll层之间的精子,以排除生精细胞和白细胞的污染。将纯化后精子的cDNA探针与Affymetrix全基因组芯片杂交。筛选出差异表达的基因,利用生物信息学方法对该基因进行分析。用RT-PCR验证该基因在正常男性和弱精患者精液精子中的差异表达,并用RT-PCR方法分析该基因在人不同组织(心、肝、脾、肺、肾、胃、脑、睾丸)中的表达。结果分析芯片杂交结果 ...
李如凯, 郭龙华
doaj
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doaj
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Zhu XF.
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