【目的】探讨体外诱导成人骨髓间质干细胞(humanmesenchymalstemcells,hMSC)向神经元样细胞分化过程中分化与凋亡的关系,为进一步研究延长神经元样细胞的存活时间提供基础。【方法】从成人骨髓分离MSC,培养扩增。以β-巯基乙醇和参芪液诱导分化,于诱导后1、3、5h分别进行免疫组化鉴定神经元烯醇化酶(NSE)、神经丝蛋白(NF)、胶质纤维酸性蛋白(GFAP)的表达,计数神经元样细胞,计算分化率。采用TUNEL方法分别检测诱导后1、3、5h的细胞凋亡率,并分析两者是否相关。【结果 ...
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