【目的】 建立适于人表皮组织的双向电泳技术,并应用质谱技术对人表皮组织蛋白质组进行初步分析?【方法】 利用组织匀浆法制备人表皮组织总蛋白?固相pH 梯度胶条进行双向电泳?PDQuest 7.4软件比较和分析电泳图谱,利用质谱技术鉴定部分蛋白斑点?【结果】 成功建立了适用于人表皮组织蛋白质组分析的双向电泳技术,获得和比较了人表皮组织双向电泳四种染色图谱?人表皮组织的蛋白质在pH5~8范围内分布均匀,分子量集中在15~100 ku之间?银染图谱获得的蛋白斑点数为586±14 ...
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Liu L, Xiao XH, Fang JH, Li GK.
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Zhan XB, Qin Y, Yan Y, Zhou J, Zhao LS.
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