Development in detection of enterohemorrhagic Escherichia coli O 157 and H 5 subtype avian influenza virus using real-time fluorescence PCR [PDF]
实时荧光PCR技术具有高灵敏度、高特异性、无需PCR反应后处理步骤等优点,近年来被广泛应用于分子生物学研究领域,尤其在临床检验方面,该技术提供了有力的支持。针对日益完善的实时荧光PCR技术和种类越来越多的荧光探针技术,本研究设计了一种新型的荧光探针——等长双链荧光探针,又称Y型荧光探针,并围绕该关键技术展开了研究。研究工作主要包括Y型荧光探针的设计组合,应用Y型荧光探针对常见的病原微生物进行实时定量的检测。文章分为两部分。在第一部分,首先,我们对不同组合的Y型荧光探针进行了筛选、优化 ...
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