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Development in detection of enterohemorrhagic Escherichia coli O 157 and H 5 subtype avian influenza virus using real-time fluorescence PCR [PDF]

open access: yes, 2005
实时荧光PCR技术具有高灵敏度、高特异性、无需PCR反应后处理步骤等优点,近年来被广泛应用于分子生物学研究领域,尤其在临床检验方面,该技术提供了有力的支持。针对日益完善的实时荧光PCR技术和种类越来越多的荧光探针技术,本研究设计了一种新型的荧光探针——等长双链荧光探针,又称Y型荧光探针,并围绕该关键技术展开了研究。研究工作主要包括Y型荧光探针的设计组合,应用Y型荧光探针对常见的病原微生物进行实时定量的检测。文章分为两部分。在第一部分,首先,我们对不同组合的Y型荧光探针进行了筛选、优化 ...
于广福
core  

[The status of ENT health care workers at the forefront of fighting against COVID-19 in Wuhan and some response options]. [PDF]

open access: yesLin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi, 2020
Zhang Y   +9 more
europepmc   +1 more source

[Conducting clinical studies during the epidemics of communicable diseases: perspectives of methodology and health economics]. [PDF]

open access: yesNan Fang Yi Ke Da Xue Xue Bao, 2020
Gong M   +10 more
europepmc   +1 more source

[Laboratory testing techniques for SARS-CoV-2]. [PDF]

open access: yesNan Fang Yi Ke Da Xue Xue Bao, 2020
Qiu F   +6 more
europepmc   +1 more source

Responding to avian influenza in poultry farms in Victoria, Australia. [PDF]

open access: yesBull World Health Organ
Blaney K   +16 more
europepmc   +1 more source

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