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[Effects of mitochondrial transplantation on full-thickness skin defects in diabetic rats]. [PDF]

open access: yesZhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Li YQ   +7 more
europepmc   +1 more source

基于DENV-prM/E基因构建DENV-VLP及其免疫学特性

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2014
摘 要:【目的】 构建和表达登革病毒4型病毒样颗粒,初步研究其免疫学特性。【方法】 用RT-PCR法扩增DENV4-prM/E基因,构建重组质粒pGAPZ-sprM/E-D4,转化X33酵母菌株,SDS-PAGE、WesternBlot鉴定目的蛋白表达。切片电镜检测病毒样颗粒,蔗糖密度梯度离心纯化。免疫三组Balb/C小鼠,每组15只。采用间接ELISA检测抗原特异性抗体,间接免疫荧光法检测免疫血清与天然病毒粒子的结合,流式细胞术分析小鼠脾淋巴细胞表型,酶联免疫斑点法(ELISPOT)检测脾分泌IFN ...
doaj  

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