Results 101 to 110 of about 6,833 (148)

广州管圆线虫抗原 IF 的体外表达系统的建立和分析

Zhongshan Daxue xuebao. Yixue kexue ban, 2006
【目的】建立广州管圆线虫特异性抗原中间纤维( IF) 基因的体外表达系统, 研究 IF 蛋白的结构和功能关系。【方法】将 IF 基因亚克隆入原核表达载体 pET- 30a- c(+)并在原核中诱导表达, Western blot 鉴定融合表达产物的免疫特性, 用组氨酸( His) 亲合层析柱分离纯化表达产物, 鉴定表达体系效率。生物信息学分析目的蛋白结构和功能关系。【结果】抗原基因 IF 在原核中与 6 个组氨酸进行了融合表达, 以可溶性表达为主, 并能在该系统中大量、稳定表达, 其相对分子质量 Mr =
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[The application of nanoparticles in the treatment and prevention of pathological scars]. [PDF]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Huang Y, Zhao L, Jin Z, Cui A.
europepmc   +1 more source

[Mesothelial impairment induced by peritoneal dialysis and its potential protective strategies]. [PDF]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Ban Y, Mei Y, Chen B, Tang W, Kang H.
europepmc   +1 more source

[Tibetan Medicine Classic Formula Srolo Bzhtang Granules Ameliorates Pulmonary Fibrosis via Dual Pathways of Nrf2/HO-1 and PI3K/AKT/mTOR Regulating Oxidative Stress]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
DU J, Baimalazong, Dejibaizhen, Basangdeji, Bianbaciren, Ye B.   +5 more
europepmc   +1 more source

CircSLC8A1_005通过编码蛋白抑制心肌成纤维细胞纤维化表型的作用

Zhongshan Daxue xuebao. Yixue kexue ban
目的探究环形RNA circSLC8A1_005调控心肌成纤维细胞纤维化表型的作用及可能机制。方法利用腺病毒介导在小鼠心肌成纤维细胞（mCFs）中过表达circSLC8A1_005，并检测mCFs中纤维化相关因I型胶原α1链（Col1a1）、Ⅲ型胶原α1链（Col3a1）和平滑肌肌动蛋白α2（Acta2）基因表达，通过EdU和划痕实验检测不同干预对mCFs的增殖和迁移能力的影响。双萤光素酶报告基因实验检测circSLC8A1_005包含的潜在核糖体进入序列（IRES）的活性。通过Western ...
胡雅婷, 高原, 伍华燕, 梁俣, 李晖, 徐金东, 刘宇鹏, 单志新   +7 more
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[<i>Huoxue Qingjie Ling</i> alleviates bile duct ligation-induced hepatic fibrosis in rats by regulating the Sirt1-autophagy signaling pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Li C, Cui L, Cao J, Fan Y, Zhou X, Zhang S, Zuo Y.   +6 more
europepmc   +1 more source

[Research progress on the quercetin target role and signaling pathway in anti-liver fibrosis]. [PDF]

Zhonghua Gan Zang Bing Za Zhi
Zhao RQ, Liu HY, Yan JM, Hao Y, Ding YX, Zhang F, Zhou RM, Zhao R, Bai F, Bai WF.   +9 more
europepmc   +1 more source

[Spatial omics in pulmonary fibrosis: advancing mechanistic insights and therapeutic strategies]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Peng Y, Xie Y, Yang F, Qiao X, Tian Y.
europepmc   +1 more source

[Silybin inhibits fibrosis after glaucoma filtration surgery in rabbits by promoting fibroblast autophagy]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhang Y, Shen Y, Tong X, Duan Y, Luo Y, Guo W.   +5 more
europepmc   +1 more source

[Role and mechanism of Wnt9a in human and mouse chronic wound healing]. [PDF]

Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Jia YH, Yuan YX, Hu DH, Guan H.
europepmc   +1 more source