Results 101 to 110 of about 7,792 (148)

洛铂抑制膀胱癌细胞增殖及诱导凋亡机制的研究

Zhongguo shiyan zhenduanxue, 2016
目的研究洛铂对人膀胱癌细胞的抑制作用,并揭示其可能的作用机制。方法体外常规培养人膀胱癌细胞株5637。利用MTT细胞增殖法检测洛铂的细胞毒性;采用Annexin V-FITC/PI凋亡试剂盒在流式细胞仪上检测癌细胞的凋亡程度;通过伤口愈合实验观察细胞的迁移;通过Transwell小室测定评估洛铂对5637细胞侵袭和迁移能力的影响;利用蛋白免疫印迹法检测细胞凋亡基因的表达。结果洛铂抑制人膀胱癌细胞并诱导其凋亡,下调膀胱癌5637细胞Survivin和Bcl-2的表达水平,上调其Bax和Caspase ...
王丰, 于倩, 王华
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IRAK-4基因沉默对人成骨样细胞凋亡的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2013
【目的】研究siRNA沉默白细胞介素-1受体相关激酶-4(IRAK-4)基因对人成骨样细胞株MG63凋亡及凋亡相关基因Bcl-2、Bax表达的影响。【方法】 以Lipofectamine 2000为载体将IRAK-4-siRNA转染入MG63细胞(CON组:不加入任何转染试剂;SC组:以scrambled siRNA序列进行转染;KD组:以特异性IRAK-4-siRNA序列进行转染),应用流式细胞术及TUNEL法检测细胞凋亡水平,western blotting检测Bcl-2、Bax基因的表达。【结果】
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[Mechanism of Trifolin in Attenuating Hypertension-Induced Renal Cell Apoptosis via Modulation of the MAPK Signaling Pathway]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Wu M, Guo Z, Xie Y, Liu H, Chen H, Lin X, Chen R, Shen A, Peng J.   +8 more
europepmc   +1 more source

Research progress in apoptosis of vascular smooth muscle cells in atherosclerosis. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2021
Yang S, Wang J, Tong L, Qi L, Chen X, Cai L.   +5 more
europepmc   +1 more source

[Elevated expressions of GRP78/CHOP in lupus nephritis: their diagnostic value and association with PERK/IRE1α pathway-mediated renal cell apoptosis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Wang Y, Zhang W, Fang T, Xie Z, Fan Y, Wang X.   +5 more
europepmc   +1 more source

[Shikonin induces hepatocellular carcinoma cell apoptosis by suppressing PKM2/PHD3/HIF-1α signaling pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao, 2023
Zhang H, Chen Z, Zhao X, Huo Q, Cheng X.
europepmc   +1 more source

[Oxidative Stress Induced by High Glucose Aggravates Immunosuppression in Diabetes Mellitus by Promoting Effector T Cell Apoptosis and Regulatory T Cell Differentiation]. [PDF]

Sichuan Da Xue Xue Bao Yi Xue Ban
Ma X, Li Z, Chen W, Zhang W, Zhang D.
europepmc   +1 more source

[Niranthin ameliorates Crohn's disease-like enteritis in mice by inhibiting intestinal epithelial cell apoptosis and protecting intestinal barrier <i>via</i> modulating p38/JNK signaling]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Tao L, Chen Y, Huang L, Zheng W, Song X, Xiang P, Hu J.   +6 more
europepmc   +1 more source

苯甲酸钠诱导ＰＣ１２细胞凋亡及对ＴＨ表达的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2014
摘 要： 【目的】 观察苯甲酸钠（ＳＢ）对ＰＣ１２肾上腺嗜铬细胞瘤细胞凋亡的诱导作用及对酪氨酸羟化酶（ＴＨ）表达的影响。【方法】 以大鼠ＰＣ１２细胞为多巴胺能神经元细胞模型，以１０～ ５０ ｍｍｏｌ／Ｌ不同浓度的ＳＢ加入ＰＣ１２细胞，培养２４ ｈ后，噻唑蓝（ＭＴＴ）比色法检测细胞活性；ＤＡＰＩ染色观察细胞凋亡形态；流式细胞术检测细胞凋亡状况以及用Ｗｅｓｔｅｒｎｂｌｏｔ检测ＴＨ蛋白表达。【结果】 ＳＢ可呈浓度依赖性抑制ＰＣ１２细胞活性，并诱导ＰＣ１２细胞核形态呈凋亡改变；流式细胞术检测显示随着ＳＢ浓度增加，
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HDAC1 overexpression inhibits steroid-induced apoptosis of mouse osteocyte-like MLO-Y4 cells by inducing SP1 deacetylation. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhang S, Lu M, Kuang G, Xu X, Fu J, Zeng C.   +5 more
europepmc   +1 more source