Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 探讨IgA肾病患者血清IgA1对足细胞凋亡的影响。 【方法】 Jacalin 亲和层析和Sephacryl S-200 分子筛用来纯化蛋白,单体IgA1(mIgA1)热聚合为聚合体IgA1(aIgA1), 同步化的足细胞与患者和健康对照来源的aIgA1共培养,流式细胞仪评价细胞凋亡情况,Real time PCR 检测Bcl-2, Bax, Fas and Fas-L mRNA表达情况。 【结果】 IgAN患者的aIgA1可诱导足细胞的凋亡,其凋亡率显著高于对照组[(30.5 ± 5.4 ...
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Zhongguo shiyan zhenduanxue, 2016 目的研究洛铂对人膀胱癌细胞的抑制作用,并揭示其可能的作用机制。方法体外常规培养人膀胱癌细胞株5637。利用MTT细胞增殖法检测洛铂的细胞毒性;采用Annexin V-FITC/PI凋亡试剂盒在流式细胞仪上检测癌细胞的凋亡程度;通过伤口愈合实验观察细胞的迁移;通过Transwell小室测定评估洛铂对5637细胞侵袭和迁移能力的影响;利用蛋白免疫印迹法检测细胞凋亡基因的表达。结果洛铂抑制人膀胱癌细胞并诱导其凋亡,下调膀胱癌5637细胞Survivin和Bcl-2的表达水平,上调其Bax和Caspase ...
王丰, 于倩, 王华
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Expression of PGRMC1 in patients with polycystic ovary syndrome and its molecular mechanism for regulating ovarian granulosa cell apoptosis and glucolipid metabolism. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2023 Zhou J +5 more
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2013 【目的】研究siRNA沉默白细胞介素-1受体相关激酶-4(IRAK-4)基因对人成骨样细胞株MG63凋亡及凋亡相关基因Bcl-2、Bax表达的影响。【方法】 以Lipofectamine 2000为载体将IRAK-4-siRNA转染入MG63细胞(CON组:不加入任何转染试剂;SC组:以scrambled siRNA序列进行转染;KD组:以特异性IRAK-4-siRNA序列进行转染),应用流式细胞术及TUNEL法检测细胞凋亡水平,western blotting检测Bcl-2、Bax基因的表达。【结果】
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[Influence of family with sequence similarity 134, member B-mediated reticulophagy on lipopolysaccharide-induced apoptosis of mouse dendritic cells]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi, 2023 Duan Y +6 more
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[Role and mechanisms of FoxO3a-related signaling pathways in breast cancer cell apoptosis]. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue BanWang Z +5 more
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Research progress in apoptosis of vascular smooth muscle cells in atherosclerosis. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2021 Yang S +5 more
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[Mechanism of Trifolin in Attenuating Hypertension-Induced Renal Cell Apoptosis via Modulation of the MAPK Signaling Pathway]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanWu M +8 more
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[High expression of MYH9 inhibits apoptosis of non-small cell lung cancer cells through activating the AKT/c-Myc pathway]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2023 Liu F, Peng L, Xi J.
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[Elevated expressions of GRP78/CHOP in lupus nephritis: their diagnostic value and association with PERK/IRE1α pathway-mediated renal cell apoptosis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoWang Y +5 more
europepmc +1 more source