Results 61 to 70 of about 5,792 (101)

苯甲酸钠诱导ＰＣ１２细胞凋亡及对ＴＨ表达的影响

Zhongshan Daxue xuebao. Yixue kexue ban, 2014
摘 要： 【目的】 观察苯甲酸钠（ＳＢ）对ＰＣ１２肾上腺嗜铬细胞瘤细胞凋亡的诱导作用及对酪氨酸羟化酶（ＴＨ）表达的影响。【方法】 以大鼠ＰＣ１２细胞为多巴胺能神经元细胞模型，以１０～ ５０ ｍｍｏｌ／Ｌ不同浓度的ＳＢ加入ＰＣ１２细胞，培养２４ ｈ后，噻唑蓝（ＭＴＴ）比色法检测细胞活性；ＤＡＰＩ染色观察细胞凋亡形态；流式细胞术检测细胞凋亡状况以及用Ｗｅｓｔｅｒｎｂｌｏｔ检测ＴＨ蛋白表达。【结果】 ＳＢ可呈浓度依赖性抑制ＰＣ１２细胞活性，并诱导ＰＣ１２细胞核形态呈凋亡改变；流式细胞术检测显示随着ＳＢ浓度增加，
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