标记21和13号染色体双色FISH探针及其在快速产前诊断中的应用
【目的】 采用细菌人工染色体(BAC)克隆分别建立21和13号染色体的直标型荧光原位杂交(FISH)探针,并分析其在产前快速诊断21/13号染色体数目异常的可行性65377;【方法】 通过生物信息学网站挑选位于21q22.2(DSCR)和13q14(RB基因)的BAC克隆,培养并提取DNA后采用切口平移法进行红/绿色(21/13)荧光的直接标记65377;取上述探针与中期细胞进行杂交,鉴定探针的杂交位置65377;取10例羊水标本分别采用标记的FISH探针(实验组)与商品化探针(对照组)进行检测 ...
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