Pathogenic characteristics and drug resistance spectrum of Enteroaggregative Escherichia coli in infant food in Liaoning Province from 2018 to 2020 [PDF]
, 2023 ObjectiveTo give a solid foundation for the epidemiological data of diarrheagenic Escherichia coli （DEC）， and provide a basis for the rational drug use for foodborne diseases caused by DEC， the continuous monitoring of DEC from 2018 to 2020 in Liaoning ...
DIAO Wenli +6 more
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Distribution of blood culture pathogens and clinical characteristics in children with acute leukemia complicated with septic shock [PDF]
, 2022 Objective To explore the distribution of blood culture pathogens and clinical characteristics in children with acute leukemia complicated with septic shock.
Liu Tinghua, Lei Jiaying, Mai Yougang
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Contamination and mobile colistin resistance gene analysis for Salmonella isolated from retail chickens in Ji'nan [PDF]
, 2023 ObjectiveTo investigate the contamination of Salmonella in retail chickens in Ji’nan city from 2020 to 2021， and to explore the prevalence of the mobile colistin resistance gene （mcr） among these Salmonella isolates.MethodsFrom December 2020 to November ...
HU Yujie +7 more
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Research on the Diversity of Insecticidal Proteins Derived from Microorganisms [PDF]
, 2021 Insect problem has been one of the most severe problems in agriculture and forestry, which causes great economic losses to the primary industry in China. For a long time, the use of chemical insecticides for insect control has led to many issues, such as
FAN Huan +7 more
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Physicochemical Characteristics and Spoilage Bacteria of Modified Atmosphere Packaged Braised Chicken during Refrigeration [PDF]
, 2023 In order to explore dynamic changes in the physicochemical characteristics and microbial diversity of modified atmosphere packaged braised chicken during refrigeration, high-throughput sequencing technology and traditional isolation and identification ...
ZHANG Li, MA Yunhao, WANG Ying, XU Baocai
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Research Progress on the Formation Mechanism of Protein/Essential Oil-based Composite Films and Application in Food Preservation [PDF]
, 2023 It has great significance to maintain the natural state of food and reduce the environmental pollution that use of bio-based packaging materials to research and development food packaging, because it has been unable to meet the demand of consumers for ...
Changbo SHI +4 more
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嗜虫耶尔森氏菌HlyA及HasA外分泌表达系统的构建 [PDF]
, 2019 【目的】构建一株具备外分泌蛋白功能的工程菌,解决杀虫毒素无法由胞内分泌至胞外,无法直接作用于虫体等问题,为松墨天牛防治提供新思路。【方法】本研究先测定从松墨天牛肠道及其生境中分离出的嗜虫耶尔森氏菌(CSLH88)的生长特性及抗性,进而对其进行分子改造。构建HlyA (pGHKW2)以及HasA (pGHKW4)外分泌表达载体,利用电穿孔法将其转入CSLH88菌株,获得能够表达绿色荧光蛋白的工程菌。利用稀释涂板及荧光体式镜检测技术对两个质粒进行遗传稳定性检测,并采用SDS-PAGE及Western ...
余云钦 +7 more
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血流感染的耐碳青霉烯类鲍曼不动杆菌的分子机制及临床研究 [PDF]
, 2018 目的探讨血流感染的耐碳青霉烯类鲍曼不动杆菌（CRAB）耐药机制,分析菌株同源性及患者的临床特征,为CRAB的感染控制提供实验依据。方法采用Bact/Alert 3D全自动血培养系统进行血培养,细菌鉴定和药敏使用Vitek-2 Compact全自动微生物分析系统。对2010年6月—2016年5月临床血流感染患者分离到的27株碳青霉烯类药物（亚胺培南和/或美罗培南MIC≥16μg/m L）耐药的CRAB复苏菌株,重新采用Vitek-MS质谱分析仪鉴定,采用改良Hodge试验检测碳青霉烯酶 ...
徐和平 +6 more
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, 2017 脓毒血症是因感染导致的一种重度炎症性反应综合征（severe inflammatory response syndrome,SIRS）,目前普遍认为脓毒血症并发急性肾衰是因SIRS发生阶段发生氮质产物潴留、代谢性酸中毒,且在病情进展成严重脓毒血症时发生内环境紊乱而致[1]。临床药学服务的要点在于协助医师尽早控制病人的全身感染 ...
王芳芳, 许小鑫, 费燕, 黄文辉
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酪氨酸激酶抑制药GW2974诱导乳腺癌细胞BT474耐药的代谢机制研究 [PDF]
, 2019 目的通过比较酪氨酸激酶抑制药GW2974给药后母本细胞株BT474与耐药株r BT474之间代谢相关因子mRNA表达的差异,探讨r BT474可能的耐药机制。方法收集对数生长期r BT474和BT474细胞,接种于96孔板,加入含有GW2974的培养基,分别于孵育0,12,24和48 h后,通过噻唑蓝法检测BT474与r BT474细胞的增殖情况。用逆转录实时定量-聚合酶链反应检测12种相关代谢因子葡萄糖转运体4(GLUT4)、2,6-二磷酸果糖激酶(PFK-2)、丙酮酸激酶2(PKM2)、乳酸脱氢酶 ...
吴光华 +6 more
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