IL-12 、LPS 对肾小管上皮细胞中lck/P38信号传递的影响 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】探讨IL-12 、LPS 对肾小管上皮细胞中lck 基因表达及活性改变的影响, 明确lck/P38信号传递途径在炎症过 程的作用。【方法】用原位杂交、放射自显影分别检测lck 基因表达及lck 活性, 用免疫印迹法对肾小管上皮细胞在IL-12、LPS 刺激下其P38磷酸化进行测定。【结果】肾小管上皮细胞经IL-12, LPS 刺激后lck mRNA 表达水平增高, lck 活性在刺激后5 min 时最强, 加入lck 抑制剂PP1 , IL-12 刺激lck 活性明显减弱。IL-12 ...
李清刚 +3 more
doaj +2 more sources
Study on the protective effect of mitochonic acid-5 on septic acute kidney injury and the underlying mechanism [PDF]
Linchuang shenzangbing zazhi目的探讨线粒体酸5（mitochonic acid-5，MA-5）是否可以减轻脂多糖（lipopolysaccharide，LPS）诱导的脓毒症急性肾损伤以及可能的机制。方法细胞实验：将肾小管细胞分为正常组、MA-5（10 µmol/L）组、LPS（10 mg/L）刺激组和LPS+MA-5治疗组，通过细胞计数试剂盒8法评估细胞活力、流式细胞术评估细胞凋亡率；活性氧（reactive oxygen species，ROS）检测试剂盒检测ROS含量；三磷酸腺苷（adenosine triphosphate ...
Li Peng-yan, Yan miao, Yang Ding-ping
doaj +3 more sources
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨白蛋白对近端肾小管上皮细胞（PTCs）增殖与凋亡的影响。【方法】用不同剂量（0．1，0．5，1，5，10，30mg／mL）去脂牛血清白蛋白（dBSA）超载体外培养的NRK52E，dBSA 0mg／mL作为对照。细胞增殖活性用MTS比色法检测和显微镜观察。细胞凋亡检测：荧光染料DAPI染核，DNA梯带实验，原位末端标记（TUNEL）实验和苔盼蓝染色计数。【结果】高剂量dBSA超载未完全融合NRK52E细胞144h后细胞变得更密集。MTS比色结果发现，高剂量dBSA（5、10、30mg／mL ...
孙良忠, 岳智慧, 陈述枚
doaj +2 more sources
Protective Effect of Freeze-Dried Fruit Powder of Phyllanthus emblica L. on Acute and Chronic Hyperuricemia and Liver and Kidney Injuries in Mice [PDF]
, 2023 Objective: To explore the protective effect of the freeze-dried fruit powder of Phyllanthus emblica L. (PEFP) against acute and chronic hyperuricemia (HUA) and liver and kidney injuries in mice.
LI Ruiting, HE Wei, SONG He, XIE Xiaoting, WU Jie, JIANG Yan
core +1 more source
Zhejiang Daxue xuebao. Lixue ban, 2008 利用透射电镜对日本沼虾触角腺的体腔囊、迷路、原肾管和膀胱的超微结构进行了研究.体腔囊由足细胞和内皮细胞组成，其中足细胞的三级足状突起分支细而多，并密布于裂隙膜上，足细胞及其足状突起之间可见隔壁连接，内皮细胞与开放式血腔相临、沿基底膜有许多窗孔.迷路细胞游离端具长而发达的微绒毛，基底及两侧质膜内陷发达，并具丰富的长杆状线粒体.原肾管细胞的微绒毛短少而零乱，基底及两侧质膜内陷更为发达，原肾管细胞之间存在有中间连接和隔壁连接.研究结果证实：日本沼虾触角腺的体腔囊、迷路和原肾管的超微结构与哺乳动物肾单位的肾小球、
ZHOUShuang-lin(周双林) +1 more
doaj +1 more source
Application status of cell models in clinical nutrition research [PDF]
, 2020 Traditional clinical nutrition research mostly stay at the stage of human and animal experiments. Cell model experiment has gradually become an important way in clinical nutrition research nowadays.
Jian HUANG +4 more
core +1 more source
Renal Protective Effects of Lanzhou Lily (Lilium davidii var. unicolor) Polysaccharide and Aerobic Exercise in Streptozotocin-Induced Diabetic Rats [PDF]
, 2023 objective: To investigate the protective effect of Lanzhou lily polysaccharide (LLP) and aerobic exercise (AE) on the kidney in a rat model of diabetic mellitus (DM).
LI Shouhan, GAO Wenfeng, CUI Zhijia
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泽黄颗粒及其拆方对糖尿病大鼠肾脏AQP1表达的调节效应 [PDF]
Objective: To observe the relationship between the expression of aquaporin-1 (AQP1) and the abnormal fluid metabolism in diabetic nephropathy rats and to explore the preventive and therapeutic mechanism of Zehuang Granule and its disassembled ...
Guo, Liang-feng +8 more
core +2 more sources
The effect of pentosan polysulfate on diabetic nephropathy renal tubular epithelial cells injury through regulating p38 MAPK signaling pathway [PDF]
, 2016 目的：观察戊糖多硫酸钠是否通过调控P38MAPK干预糖尿病肾病肾小管上皮细胞的损伤。方法：利用不同浓度（10mmol/L～50mmol/L）葡萄糖刺激肾小管上皮细胞（HK-2）48h，应用CCK-8比色法检测肾小管上皮细胞增殖的改变。利用不同时间段（0h～48h）葡萄糖刺激细胞，用Westernblot法检测P38MAPK蛋白的表达水平。分为正常对照组（control组）、高糖组（HG组，葡萄糖30mmol/L)、戊糖多硫酸钠（PPS）组（PPS200µg/mL)、高糖＋戊糖多硫酸钠组（HG＋
张天英
core
Role of tubular cell-derived exosomes in renal ischemia reperfusion injury [PDF]
Renal ischemia reperfusion injury refers to a pathophysiological phenomenon that aggravates the degree of renal injury due to the restoration of blood supply after transient ischemia of renal tissue.
Ai-sha Zhang +7 more
core +1 more source