RNAi干扰WT1和Pax2表达对逆转肾小管上皮间充质转化的作用
Zhongshan Daxue xuebao. Yixue kexue ban, 2011 【目的】 探讨抑制肾小管上皮间充质转化(EMT)过程中胚胎发育关键基因WT1和Pax2的表达对EMT的逆转作用【方法】 采用RNAi技术分别抑制WT1和Pax2分别构建pshRNA-WT1和pshRNA-Pax2表达载体,采用脂质体转染技术,使质粒瞬时转染NEK52E细胞后用10 ng/mL IL-1α刺激细胞,分别提取不同时间点细胞的RNA和蛋白质,采用RT-PCR和Western blot检测细胞WT1Pax2Snail上皮细胞标志E-cadherin和间充质标志α-SMA的mRNA和蛋白质的表达 ...
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[Clinical efficacy and safety of rituximab in treating renal injury in primary Sjögren syndrome]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanZhao Y +6 more
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2015 摘 要： 【目的】 睾丸肾上腺残余肿瘤（ＴＡＲＴ）是睾丸少见良性肿瘤，因其罕见常易误诊为睾丸间质细胞瘤等其他肿瘤，是临床诊断难点。本研究收集并总结了１３例ＴＡＲＴ的病理临床特点，目的是提高对该病的认识和临床诊断水平。【方法】 收集中山大学附属第一医院２００９－２０１４年间１３例确诊的ＴＡＲＴ病例，通过回顾影像学资料和病理组织学特点、免疫组化染色、结合临床症状预后分析，归纳ＴＡＲＴ的临床病理特点。【结果】 １３例ＴＡＲＴ患者均为男性，平均年龄１２．１５岁，临床均表现为肾上腺皮质综合症（ＣＡＨ ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 研究高糖诱导肾小管上皮细胞肥大的作用途径及可能机制65377;【方法】 HK-2细胞分正常对照组(培养基含葡萄糖1 g/L),等渗对照组(葡萄糖1 g/L + 甘露醇3.5 g/L)和高糖组(葡萄糖4.5 g/L)培养65377;收集各组培养至24 h,48 h,96 h的细胞,检测结缔组织生长因子(CTGF)65380;p27kip的mRNA水平65380;CTGF65380;p27kip的蛋白水平65380;细胞增殖活力65380;细胞周期分布及细胞总蛋白含量65377 ...
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[Advances on efficacy and mechanisms of acid fibroblast growth factor for promoting injured tissue repair]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiZhu F +5 more
europepmc +1 more source
[Research progress on the correlation between Akkermansia muciniphila and cardiovascular diseases]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoJiang S, Gong L, Zheng H.
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[<i>Danzhi Jiangtang</i> Capsule improves renal vascular endothelial function in rats with diabetic nephropathy by downregulating the Notch1/NICD/MAML1 signaling pathway]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoZhu S +7 more
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[Ultra-early administration of eculizumab in a child with atypical hemolytic uremic syndrome: a case report]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiGuo DD +4 more
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[A case of segmental atrophy of the liver in children]. [PDF]
Zhonghua Gan Zang Bing Za ZhiQiu YL, Mao Y, Lu CL.
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[<i>Shenqi Xiezhuo</i> Decoction alleviates renal fibrosis in rats by ameliorating oxidative stress and inflammation through the Rap1/MAPK/FoxO3a signaling pathway]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLu X +6 more
europepmc +1 more source