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抗原致敏IL-12基因修饰的树突状细胞诱导抗肾癌免疫的体外研究 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨肾癌细胞抗原致敏白介素-12（IL-12）基因修饰的树突状细胞（DC）体外诱导、激活细胞毒性T淋巴细胞（CTL）免疫杀伤肾癌细胞的效能及相关免疫机制。【方法】采用重组逆转录病毒介导IL-12基因修饰人外周血单个核细胞（PBMC）来源的DC；超声细胞破碎法提取肾癌细胞粗提抗原（Ag）致敏经IL-12转染的DC；ELISA法检测各组DCs和各组CTLs上清中IL-12、IFN-γ因子的分泌水平；流式细胞仪（FACS）分析各组DCs表面CD83、CD86、HLA-DR的表达 ...
罗道升 +5 more
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Advances in the research, diagnosis and treatment of renal cell carcinoma in 2022 [PDF]
, 2023 Renal cell carcinoma (RCC) is one of the three major urinary system tumors. With the changes of lifestyle and the rise of obesity, hypertension and other diseases, the incidence of RCC is increasing.
TIAN Xi, XU Wenhao, ZHU Shuxuan, AIHETAIMUJIANG•Anwaier, SU Jiaqi, YE Shiqi, QU Yuanyuan, SHI Guohai, ZHANG Hailiang, YE Dingwei
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Zhongshan Daxue xuebao. Yixue kexue ban, 2023 目的分析乳酸脱氢酶A（LDHA）在肾细胞癌组织及细胞系中的表达情况并探讨其影响肾细胞癌细胞进展的方式。方法收集自2018年6月至2022年6月在我院通过手术方式获取的肾细胞癌组织标本52例及癌旁组织标本49例，免疫组织化学法比较LDHA的表达差异。通过qRT-PCR及Western blot实验检测LDHA在正常人近端肾小管上皮细胞系（HK-2）及肾细胞癌细胞系（A498， Caki-2， ACHN， 786-O）中的表达水平。构建LDHA-shRNA重组质粒，转染至786-O以敲低LDHA表达 ...
邱文瀚 +4 more
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Preliminary application of improved 3D printed pathological section box to assisting stitching pathological images of bone tumor [PDF]
, 2023 Objective·To explore the feasibility of applying improved pathological section boxes based on image post-processing and 3D printing technology to assisting artificial intelligence (AI) in stitching pathological images of bone tumors.Methods·Bone tumor ...
AI Songtao +6 more
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Development of interleukin-21 as a therapeutic drug [PDF]
, 2017 白细胞介素21; (IL-21)是一种具有多效免疫调节活性的细胞因子,参与先天性免疫反应和适应性免疫反应的多个环节,与自身免疫性疾病、肿瘤、病毒感染等疾病的发病过; 程相关。研究表明,在动物模型中通过增强或抑制IL-21的生物学功能对多种疾病具有治疗意义,多项以重组人IL-21为受试药物的临床试验已经开展。本; 文就IL-21的生物学功能,以及IL-21作为生化药物在临床治疗中的研究进展和应用前景进行阐述。Interleukin-21 (IL-21) is a cytokine with broad ...
刘狄彤 +5 more
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Expression and clinical significance of PLA2G2A in kidney renal papillary cell carcinoma [PDF]
, 2023 Objective·To investigate the expression and clinical significance of phospholipase A2 Group ⅡA (PLA2G2A) in kidney renal papillary cell carcinoma (KIRP), and provide new ideas for seeking KIRP targets.Methods·The expression level of PLA2G2A in pan-cancer
LI Fang +5 more
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Journal of Diabetes, Volume 11, Issue 12, Page 993-1001, December 2019., 2019 Highlights Type 2 diabetes (T2D) is an independent predictor of survival and recurrence after surgery in patients with renal cell carcinoma (RCC). This finding reveals the importance of T2D in determining the prognosis of patients with RCC. Abstract Background Diabetes is a risk factor for various cancers, but its prognostic role in renal cell ...
Haosen Yang +8 more
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Zhongguo shiyan zhenduanxue, 2020 目的探讨自噬在硼替佐米诱导的肾癌细胞凋亡中的作用。方法采用MTT检测硼替佐米对人肾癌ACHN细胞增值率的影响;通过Western Blotting检测硼替佐米对人肾癌ACHN细胞凋亡相关蛋白cleaved caspase-3及自噬相关蛋白LC3表达水平的影响;联用自噬抑制剂3-MA后,MTT检测细胞增殖率,Western Blotting检测凋亡相关蛋白cleaved caspase-3表达水平。结果硼替佐米对肾癌ACHN细胞的增殖有抑制作用,并且增加人肾癌ACHN细胞凋亡相关蛋白cleaved ...
陈福东, 肖帅, 张鸿雁, 毕林涛
doaj
Zhongguo shiyan zhenduanxue, 2020 目的观察肾细胞癌中Holliday交叉识别蛋白基因和蛋白表达的改变。方法肾癌A498细胞、os-rc-2细胞作为实验组,人肾小管上皮细胞HK2作为对照组,实时荧光定量聚合酶链反应分析Holliday交叉识别蛋白在实验组和对照组的基因表达情况,蛋白印迹分析其蛋白表达情况。结果与对照组人肾近曲小管上皮细胞HK2细胞相比较,os-rc-2和A498肾癌细胞中Holliday交叉识别蛋白基因和蛋白表达水平均明显下降 ...
原江水 +3 more
doaj
Effect of bone marrow mesenchymal stem cells on nude mice xenograft tumor of renal cell carcinoma [PDF]
, 2014 目的：研究骨髓间充质干细胞(mesenchymalstemcells,MSC)对A498肾癌裸鼠移植瘤的影响。方法：培养肾癌A498细胞和人MSC，用CM-Dil标记MSC并对标记后MSC细胞的活性和增殖能力进行比较，构建A498肾癌裸鼠皮下移植瘤模型，实验裸鼠共36只，当裸鼠移植瘤长径达0.5~0.8cm，将其随机分为2组：一组注射CM-Dil标记的MSC（MSC组），另一组注射生理盐水（NS对照组），其中，每组各有6只用于观察生存期。从接种肿瘤的第1天起，隔天观察裸鼠饮食、排便 ...
张振奇
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