利用XIAP-/-成纤维细胞,检测在剔除XIAP的情况下Xaf1诱导细胞凋亡的作用,探索Xaf1诱导细胞凋亡的机制?【方法】 Xaf1诱导的XIAP-/-成纤维细胞凋亡由流式细胞DNA含量来表示,免疫荧光显微镜检测XIAP-/-成纤维细胞中Xaf1的亚细胞分布,共转染并细胞周期 DNA 含量流式细胞术检测Bcl2对Xaf1诱导的细胞凋亡的影响,细胞色素C流式细胞术检测Xaf1对细胞色素C释放的调节?【结果】 Xaf1与TNFα显著协同诱导XIAP-/-成纤维细胞凋亡,凋亡峰值27 ...
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[Selenocystine inhibits colon cancer cell growth by promoting reactive oxygen species generation to trigger oxidative damage]. [PDF]
Song Q +8 more
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[Research Progress on the Role and Mechanisms of PYCR1 in Tumorigenesis and Progression]. [PDF]
Meng Y, Yang Z, Feng H, Li H, Li B.
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[Research advances on the targeted programmed cell death regulatory network for treatment of refractory diabetic wounds]. [PDF]
Zhang ZH, He WF.
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[Advances in research on the mechanisms of reactive oxygen species in platelet activation and thrombosis]. [PDF]
Shen RY, Xu KL, Qiao JL, Fu P.
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[Gastrodin inhibits ferroptosis to alleviate hypoxic-ischemic brain damage in neonatal mice by activating GPX4/SLC7A11/FTH1 signaling]. [PDF]
Guo T +8 more
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Professional Committee Of Spine And Spinal Cord Injury Rehabilitation China Association Of Rehabilitation Of Disabled Persons +3 more
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[Role and mechanism of ubiquitin-specific protease 35 in ferroptosis of rheumatoid arthritis-fibroblast like synoviocytes]. [PDF]
Feng L, Hong L, Chen Y, Cai X.
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[Bifunctional biomaterials: An innovative strategy for synergistic bone tumor therapy and bone regeneration]. [PDF]
Feng W, Chang B, Wang C, Fan B.
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基于NOD样受体热蛋白结构域相关蛋白3炎症小体信号筛选猴痘病毒调控炎症的关键蛋白
目的筛选并鉴定猴痘病毒(MPXV)调控NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎症小体信号的关键蛋白。方法以MPXV国内流行毒株的基因组为模板,构建可能具有炎症调控功能的病毒基因表达质粒库(共82个)。将NLRP3、凋亡相关斑点样蛋白(ASC)、半胱氨酸蛋白酶-1(Caspase-1)、含有荧光素酶报告基因的白细胞介素1β前体(pro-IL-1β-Luc)蛋白的真核表达质粒以及病毒基因表达质粒共转染HEK293T细胞,通过检测细胞上清液中的荧光素酶活性来监测NLRP3炎症小体信号通路的活化 ...
罗铭兴 +6 more
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