Results 11 to 20 of about 970,839 (126)
绿色荧光蛋白基因 gfp 真核表达载体 pcTKGFP 的构建 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 1999 目的: 构建携带绿色荧光蛋白基因( gfp)的真核表达载体 pcTKGFP,为利用绿色荧光蛋白标记在实验动物体内进 行自杀性基因治疗的研究和转基因动物打靶载体的构建提供基因材料。方法: 采用 PCR 技术从商品质粒 pEGP -C1 中扩增出 gfp( 799 bp) ,PCR 产物用 XbaⅠ和 BamH Ⅰ双酶切后定向克隆至真核表达载体 pcTK, 重组质粒 pcTKGFP 用限制性内切酶和 DNA 序列分析进行鉴定。结果: 部分 DNA 序列分析证明 PCR 产物为 gfp, 成功筛选到携带 ...
doaj +1 more source
Journal of Traumatic Stress, Volume 35, Issue 2, Page 581-592, April 2022., 2022 Abstract Pakistan is considered to be relatively unsafe for journalists; however, little is known about how working in situations that involve a high risk of trauma exposure and personal threat impacts journalists’ mental health. The present study aimed to examine the associations among reporting on topics that carry a high risk of trauma exposure ...
Suzanna M. Koster +3 more
wiley +1 more source
Journal of Applied Ecology, Volume 58, Issue 11, Page 2566-2578, November 2021., 2021 The development of fungicide resistance greatly threatens food security and ecological sustainability, and it is urgent need to develop agricultural practices which can ameliorate this problem. Our results show that potato crop with a genetic diversity is associated with a late blight pathogen of higher fungicide sensitivity and lower potential of ...
Li‐Na Yang +8 more
wiley +1 more source
转染自杀基因 pCEAcd-tk 的 SPC-A-1 细胞的死亡机制 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】探讨自杀基因 pCEAcd-tk/前体药物体系旁观者效应的细胞死亡机制。【方法】提取质粒pCEAcd-tk,转染 到肺癌细胞株 SPC-A-1 中, 与未转染的细胞按2∶ 8 的比例混合后加到 96 孔培养板中(每孔 3×103) ,加前体药物 5-FC( 5μmol/ L)和 GCV( 10 μ mol/L)孵育 5 d 后, MTT 法测定细胞存活率而估计旁观者效应。取处理后的细胞, 提取 DNA, 琼脂糖凝胶电 泳; Hoechst 染色后荧光显微镜观察及碘化丙啶( PI)染色 ...
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Detecting deterrence from patrol data
Conservation Biology, Volume 33, Issue 3, Page 665-675, June 2019., 2019 Abstract The threat posed to protected areas by the illegal killing of wildlife is countered principally by ranger patrols that aim to detect and deter potential offenders. Deterring poaching is a fundamental conservation objective, but its achievement is difficult to identify, especially when the prime source of information comes in the form of the ...
Andrew D. M. Dobson +4 more
wiley +1 more source
Veterinary Dermatology, Volume 36, Issue 2, Page 148-158, April 2025.Background – Biofilm production by canine otitis externa (COE) pathogens and resistance development to multiple antimicrobials are commonly reported problems in veterinary practice. The use of adjuvants to disrupt biofilms may be a viable adjunctive therapy.
Bhumika F. Savaliya +3 more
wiley +1 more source
, 2009 肿瘤的自杀基因疗法是近年来肿瘤基因治疗的研究热点,是一种具有潜在临床应用前景的新的肿瘤基因治疗策略。本文就近年来自杀基因疗法在肿瘤治疗中取得的研究进展,分别从作用机制、自杀基因系统、旁观者效应 ...
颜江华, 李枚娟, 任涛
core
Wildlife Letters, Volume 2, Issue 4, Page 179-191, December 2024.We identified seven groups reflecting different patterns of engagement with wildlife, ranging from Champions to Disengaged. Outdoor recreation had the most consistent pattern of association with group membership. Respondents who were members of more engaged groups were also more likely to exhibit polarized views about wildlife management strategies ...
Cristina Romero‐de‐Diego +3 more
wiley +1 more source
Sexuality, Gender &Policy, Volume 7, Issue 4, Page 366-381, November 2024.Abstract This paper is based on a study funded by the National Office for Suicide Prevention (NOSP), exploring sex worker mental health through a qualitative study of eighteen sex workers living and working in the Republic of Ireland and with participants from service provider organizations. This paper utilizes and adapts the concept of minority stress
Paul Ryan +3 more
wiley +1 more source
Rare Metals, Volume 43, Issue 11, Page 6006-6022, November 2024.Graphical abstract AbstractCisplatin resistance is the main cause for the failure of cancer therapy. To solve the problem, we proposed to develop a novel human serum albumin (HSA) nanoplatform to integrate chemotherapy, photothermal therapy (PTT) and immunotherapy.
Xue‐Yu Man +7 more
wiley +1 more source